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Example sentences for: hek

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, head poly(A)+ RNA from fly heads that expressed dominant-negative Fos and Jun molecules; and poly(A)+ RNA from HEK293 cells that expressed either a cadherin or Tcf inhibitor of the β-catenin pathway were also collected and examined [ [ 7 8 ] ; Kamb, unpublished].
A single clone of transfected HEK cells stably expressing approximately 3 pmoles of μ opioid receptors per mg of protein, as measured by [ 3H]-DAMGO binding, was propagated and used as a model for characterization of the binding profiles of a set of opioid ligands at μ opioid receptors.
The fusion proteins were transiently expressed in HEK 293 cells [ 37 ] . Transfections were done using Lipofectamine 2000 (Gibco BRL).
Whole-cell patch clamp recording was used to test the GluR1 fusion proteins for function in transiently transfected HEK 293 cells as previously described [ 41 ] . The external solution was (in mM): 150 NaCl, 3 KCl, 2 CaCl 2 , 1 MgCl 2 , 5 glucose, 0.002 glycine and 10 HEPES (pH 7.4).
RNA for the Incyte platform experiments was poly(A)+ RNA prepared from HEK293 human cells that expressed a mutant (S45Y) β-catenin oncogene [ 11 12 ] . Other samples (e.g.