Example sentences for: hek

How can you use “hek” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A single clone of transfected HEK cells stably expressing approximately 3 pmoles of μ opioid receptors per mg of protein, as measured by [ 3H]-DAMGO binding, was propagated and used as a model for characterization of the binding profiles of a set of opioid ligands at μ opioid receptors.

  • Human embryonal kidney (HEK) cells (line 293) and NIH 3T3 mouse fibroblasts were obtained from American Type Culture Collection (Rockville, MD) and were maintained at 37°C in a 5% CO 2 atmosphere in Dulbecco's modified Eagle's medium containing 10% (v/v) fetal bovine serum.

  • 12 × 10 6HEK 293 cells were transfected, using DEAE-dextran [ 40 ] , with 25 μg of plasmid DNA.

  • BHK, HEK293, and COS-7 cells were cultured in DMEM containing 10% fetal bovine serum.

  • The 1.2 kb coding sequence of the receptor was subcloned into pCI-neo ®mammalian expression vector (Promega, Madison, WI) using the restriction sites EcoRI and Sal I. HEK 293 cell were transfected with the vector using the lipofectin ®reagent (Life Technologies, Rockville, MD).


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