Words similar to hek

Example sentences for: hek

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The 1.2 kb coding sequence of the receptor was subcloned into pCI-neo ®mammalian expression vector (Promega, Madison, WI) using the restriction sites EcoRI and Sal I. HEK 293 cell were transfected with the vector using the lipofectin ®reagent (Life Technologies, Rockville, MD).
The main advantage of using transfected HEK cells for this study is that these cells do not contain endogenous μ opioid receptors, but express the G proteins necessary for the proper coupling of the transfected genes to the respective second messenger systems.
The procedure for preparation of the adenovirus was co-transfection of a human embryonic kidney cell line (HEK 293) with the pACCMVpLpa plasmid and another plasmid (pJM17) containing most of the viral genome [ 30 ] . A relatively rare recombination of the two plasmids occurs in the HEK 293 cells that reconstitutes the adenoviral genome.
BHK, HEK293, and COS-7 cells were cultured in DMEM containing 10% fetal bovine serum.
Next, adeno-RGS3 was isolated by clonal selection in HEK 293 cells followed by a second PCR to confirm the presence of RGS3 cDNA.