Words similar to hek-
Example sentences for: hek-
How can you use “hek-” in a sentence? Here are some example sentences to help you improve your vocabulary:
EGFR down-regulation does not affect DSLET-mediated ERK activation in HEK-293 cells
In conclusion, we report that the δ-OR agonist, DSLET, induces significant ERK activation in the absence of any increase in phosphorylation of the co-expressed EGFR in HEK-293 cells.
Complete methods for the stable transfection of HEK-293 cells to express the wild type receptor can be found in a previous report [ 13 ] . Both cell lines were seeded in 6-well dishes at an initial cell density of 1 × 10 6cells/well and were maintained in Dulbecco's modified Eagle's medium (DMEM; GibcoBRL) supplemented with 2 mM L-glutamine, 5% fetal bovine serum (FBS) (Gemini Bio-Products, Calabasas, CA), and 100 μg/ml gentamycin sulfate (Gemini Bio-Products, Calabasas, CA).
Src has also been found to co-immunoprecipitate with the agonist-stimulated β 2 -AR during the period of ERK activation [ 8 ] . Our previous work [ 11 13 ] suggested that a Src-like protein tyrosine kinase was primarily responsible for tyrosine phosphorylation of the δ-OR and ERK activation induced by DTLET when the receptor was expressed in CHO or HEK-293 cells, because both responses were blocked by the presence of 50 μM PP1.
In the presence of the EGFR, ligand activation of LPA and ET-1 receptors did not increase EGFR phosphorylation even though both the LPA and ET-1 receptors stimulated ERK activity by 10-fold over basal [ 27 ] . Specifically, key protein intermediates may be physically associated with endogenous RTKs in HEK-293 cells, and therefore, down-regulation of the EGFR may negatively affect ERK phosphorylation by opioids.
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