Words similar to hek-
Example sentences for: hek-
How can you use “hek-” in a sentence? Here are some example sentences to help you improve your vocabulary:
These results suggest that DSLET utilizes a G i -coupled δ-OR to stimulate ERK 1 and 2. We then took advantage of the HEK-293 cell line's endogenous expression of the EGFR to examine this receptor's ability to activate ERKs.
Src has also been found to co-immunoprecipitate with the agonist-stimulated β 2 -AR during the period of ERK activation [ 8 ] . Our previous work [ 11 13 ] suggested that a Src-like protein tyrosine kinase was primarily responsible for tyrosine phosphorylation of the δ-OR and ERK activation induced by DTLET when the receptor was expressed in CHO or HEK-293 cells, because both responses were blocked by the presence of 50 μM PP1.
We have shown that δ-opioids increase the phosphorylation of ERKl/2 in HEK-293 cells when the mouse wild type (WT) δ-OR is stably expressed [ 11 13 ] . The concentrations required to observe DSLET-mediated ERK phosphorylation (EC 50 = 10 nM; Figure 1a) are consistent with those reported for other δ-OR-mediated events, including G protein activation and adenylyl cyclase inhibition [ 16 17 ] . Moreover, no change in total ERK expression was observed, concomitantly, with ERK phosphorylation in any of our experiments (data not shown).
Complete methods for the stable transfection of HEK-293 cells to express the wild type receptor can be found in a previous report [ 13 ] . Both cell lines were seeded in 6-well dishes at an initial cell density of 1 × 10 6cells/well and were maintained in Dulbecco's modified Eagle's medium (DMEM; GibcoBRL) supplemented with 2 mM L-glutamine, 5% fetal bovine serum (FBS) (Gemini Bio-Products, Calabasas, CA), and 100 μg/ml gentamycin sulfate (Gemini Bio-Products, Calabasas, CA).
WT-δ-OR-expressing HEK-293 cells were exposed to increasing concentrations of either DSLET (1-1000 nM) or EGF (0.
Loading...