Words similar to hcaf

Example sentences for: hcaf

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Interestingly, we found that hCCR4 also binds to the second human yPOP2 paralog, hCAF1.
The pVP16-hCAF1 and pVP16-hPOP2 constructs were obtained upon insertion of the hCAF1 and hPOP2 coding regions into the pSG5FNV vector, in-frame with the VP16 transactivation domain coding sequence, as previously described [ 17 25 ] .
Here we report the identification of nineteen yCCR4-like proteins in eukaryotes and show, by phylogenetic and genomic analyses, that they are grouped into four distinct families, one of which contains the yCCR4 orthologs : these orthologs, in animals, have conserved the yCCR4 leucine-rich repeat, and we show, using two-hybrid assays and far-Western experiments, that the human protein binds to the human yPOP2 homologs, i.e. hCAF1 and hPOP2, in a LRR-dependent manner.
To verify that hCCR4 can bind directly to hCAF1, we performed a far-Western blot analysis (Fig.
To determine whether yCCR4-yPOP2 interactions are evolutionarily conserved, we examined whether hCCR4 can bind hCAF1 and hPOP2 in a two-hybrid assay in mammalian cells.