Words similar to ha-ppt
Example sentences for: ha-ppt
How can you use “ha-ppt” in a sentence? Here are some example sentences to help you improve your vocabulary:
The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.
Cells in early log phase growth when PPT1 gene expression was maximal were used to localize HA-Ppt1p.
The relative level of HA-Ppt1p expression was determined by comparing the band intensity of HA-Ppt1p to that of Cdc28p using ImageQuant software version 5.1 (Molecular Dynamics).
Cloning, subcloning, and gene replacement for the expression of HA-Ppt1p in yeast cells
Immunoblotting showed that HA-Ppt1p levels were highest in early log phase, and then decreased dramatically during the stationary phase (Fig.
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