Example sentences for: ha-ppt

How can you use “ha-ppt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The relative level of HA-Ppt1p expression was determined by comparing the band intensity of HA-Ppt1p to that of Cdc28p using ImageQuant software version 5.1 (Molecular Dynamics).

  • Cloning, subcloning, and gene replacement for the expression of HA-Ppt1p in yeast cells

  • A strain expressing HA-Ppt1p was made using the two-step gene replacement strategy [ 39 ] . First, the upstream sequence of PPT1 (-955 to -1) was amplified from yeast genomic DNA by PCR using a 5' primer containing a Kpn I restriction site (5'-gatcGGTACCattagcacggtgccttaacca-3') and a 3' primer containing a Xho I site and HA coding sequence (5'-gatcCTCGAGatcgataccgtcgagctagcgtagtctgggacgtcgtatgggtatcgactcatATTCGAGATATTTGATTATACCAAGGC-3').

  • Cells in early log phase growth when PPT1 gene expression was maximal were used to localize HA-Ppt1p.

  • Fluorescence microscopy indicated that HA-Ppt1p was present throughout the cell, including the nucleus (Fig.


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