Example sentences for: ha-ppt

How can you use “ha-ppt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.

  • Cells in early log phase growth when PPT1 gene expression was maximal were used to localize HA-Ppt1p.

  • The relative level of HA-Ppt1p expression was determined by comparing the band intensity of HA-Ppt1p to that of Cdc28p using ImageQuant software version 5.1 (Molecular Dynamics).

  • Cloning, subcloning, and gene replacement for the expression of HA-Ppt1p in yeast cells

  • Immunoblotting showed that HA-Ppt1p levels were highest in early log phase, and then decreased dramatically during the stationary phase (Fig.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast