Words similar to ha-ppt

Example sentences for: ha-ppt

How can you use “ha-ppt” in a sentence? Here are some example sentences to help you improve your vocabulary:

A strain expressing HA-Ppt1p was made using the two-step gene replacement strategy [ 39 ] . First, the upstream sequence of PPT1 (-955 to -1) was amplified from yeast genomic DNA by PCR using a 5' primer containing a Kpn I restriction site (5'-gatcGGTACCattagcacggtgccttaacca-3') and a 3' primer containing a Xho I site and HA coding sequence (5'-gatcCTCGAGatcgataccgtcgagctagcgtagtctgggacgtcgtatgggtatcgactcatATTCGAGATATTTGATTATACCAAGGC-3').
The expression of HA-Ppt1p was detected by immunoblot using the 12CA5 monoclonal anti-HA antibody (Roche Molecular Biochemicals).
Cells in early log phase growth when PPT1 gene expression was maximal were used to localize HA-Ppt1p.
The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.
GAL1 promoter in a centromeric or episomal plasmid, respectively, indirect immunofluorescence of HA-Ppt1p or direct fluorescence from EGFP-Ppt1p showed that both proteins were also localized throughout the cell.