Example sentences for: ha-ppt

How can you use “ha-ppt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.

  • To examine when Ppt1p is expressed and where it is localized, a yeast strain (WHT4-1) was generated in which the PPT1 gene was replaced with cDNA encoding hemagglutinin-tagged Ppt1p (HA-Ppt1p).

  • The expression of HA-Ppt1p was detected by immunoblot using the 12CA5 monoclonal anti-HA antibody (Roche Molecular Biochemicals).

  • Cloning, subcloning, and gene replacement for the expression of HA-Ppt1p in yeast cells

  • Fluorescence microscopy indicated that HA-Ppt1p was present throughout the cell, including the nucleus (Fig.


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