Words similar to ha-ppt

Example sentences for: ha-ppt

How can you use “ha-ppt” in a sentence? Here are some example sentences to help you improve your vocabulary:

Immunoblotting and immunocytochemical analysis of HA-Ppt1p expression
When HA-Ppt1p or EGFP-Ppt1p was overexpressed from the
The pattern of PPT1 gene expression was identical in wild-type cells and cells expressing HA-Ppt1p, indicating that the epitope tag did not alter transcription or message stability (data not shown).
To examine when Ppt1p is expressed and where it is localized, a yeast strain (WHT4-1) was generated in which the PPT1 gene was replaced with cDNA encoding hemagglutinin-tagged Ppt1p (HA-Ppt1p).
The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.