Words similar to ha-nfatp
Example sentences for: ha-nfatp
How can you use “ha-nfatp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Upon discovering that recombinant HA-NFATp was phosphorylated in its N-terminal region we asked if the phosphorylation state of HA-NFATp would influence its ability to associate with nuclei in an in vitro assay.
This led us to hypothesize that the recombinant HA-NFATp purified from baculovirus infected insect cells was phosphorylated.
To test this hypothesis and to determine if phosphorylation of a distinct region of HA-NFATp was responsible for aberrant migration in SDS gels, we expressed and purified full-length HA-NFATp and three deletions: HA-NFATp(331-921), HA-NFATp(1-722), and HA-NFATp(331-722).
As shown in Figure 5A, the apparent molecular weight of full-length HA-NFATp was significantly decreased by the phosphatase treatment, demonstrating that recombinant HA-NFATp purified from insect cells is phosphorylated.
HA-NFATp and cJun/cFos (amounts indicated in Figure 2legend) were incubated with promoter DNA (final concentration 0.2 nM) for 15 min at 30°C under buffer conditions that were identical to those used for transcription in a reaction volume of 20 μl.