Words similar to ha-nfatp

Example sentences for: ha-nfatp

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We also found that HA-NFATp could be purified from cell extracts by DNA affinity chromatography using an immobilized double stranded oligonucleotide containing two high affinity NFAT binding sites, followed by elution with NaCl (Figure 1C).
For the preparation of HA-NFATp, Hi-five cells were infected with the HA-NFATp virus for 44 hr at 27°C.
In other studies we found the observed equilibrium dissociation constant ( obs K D ) of recombinant HA-NFATp for a high affinity NFAT site (mouse IL-4 promoter) to be 1 nM (data not shown).
Upon purifying recombinant HA-NFATp, we noticed that the protein migrated at an apparent molecular weight similar to the native NFATp in unstimulated T cells (approximately 140 kD when compared to size standards).
This led us to hypothesize that the recombinant HA-NFATp purified from baculovirus infected insect cells was phosphorylated.