Words similar to ha-pp
Example sentences for: ha-pp
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These results further support the view that induced PP1α behaves similarly if not identically to endogenous PP1α, and provides a possible mechanistic explanation for 6His-HA-PP1α localization to the nucleus.
The fusion gene was then cut from the 6His-HA-PP1α-pcDNA3 plasmid and ligated into pTEP4m, which contains a tetracycline response element for inducible expression [ 25 ] , and a hygromycin resistance gene for selection in eukaryotic cells (see Figure 1).
RT-PCR of polysome and total RNA specific for endogenous PP1α and 6His-HA-PP1α
Targeting of PP1 to the nucleus is due in part to a PP1-associated nuclear targeting subunit (PNUTS; [ 27 ] ). To further investigate the properties of inducible PP1α, we performed coprecipitation studies to determine if PNUTS can also be found associated with 6His-HA-PP1α.
Following separation by SDS-PAGE and western blotting, antibody to hemagglutinin was used to detect any associated 6His-HA-PP1α.
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