Words similar to ha-pp

Example sentences for: ha-pp

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The doxycycline-independent increase in 6His-HA-PP1α is attributed to background expression when using this system [ 25 ] . As predicted for a protein under the control of the doxycycline-inducible promoter, the abundance of 6His-HA-PP1α decreases in the absence of the inducer over time (Figure 2C).
We observed that endogenous PP1α protein levels decrease in response to increasing levels of induced 6His-HA-PP1α protein.
As expected [ 25 ] , some low-level expression of 6His-HA-PP1α in the absence of the inducer doxycycline can also be detected.
Parallel experiments performed using the same lysate and antibody to hemagglutinin for immunoprecipitation and western blotting show the position of 6His-HA-PP1α and the fact that endogenous PP1α is not recognized by this antibody (bottom panel).
The fusion gene was then cut from the 6His-HA-PP1α-pcDNA3 plasmid and ligated into pTEP4m, which contains a tetracycline response element for inducible expression [ 25 ] , and a hygromycin resistance gene for selection in eukaryotic cells (see Figure 1).