Words similar to ha-pp

Example sentences for: ha-pp

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Western blotting for additional isoforms of PP1 reveals that this reduction in PP1α following induction is isotype-specific; when 6His-HA-PP1α levels increase, concomitant with a decrease in PP1α levels, PP1δ and PP1γ1 levels remain relatively unchanged from those found in uninduced cell lysate (Figure 10).
Indeed, the abundance of endogenous PP1α RNA found in uninduced cells appears equal to or greater than the combined abundance of endogenous and 6His-HA-PP1α RNA (Figure 9), supporting the notion that some level of translational control may also exist.
If mRNA association with ribosomes is truly a measure of translation efficiency, one conclusion from these data is that in doxycycline-induced cells, 6His-HA-PP1α RNA is more efficiently translated than endogenous PP1α.
Indeed, a time course of induction followed by western blotting of whole cell lysates using antibody to PP1α revealed diminution of the endogenous PP1α signal after 4 hr of induction, while the 6His-HA-PP1α signal increased (Figure 8).
RT-PCR of polysome and total RNA specific for endogenous PP1α and 6His-HA-PP1α