Words similar to ha-pp
Example sentences for: ha-pp
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Following separation by SDS-PAGE and western blotting, antibody to hemagglutinin was used to detect any associated 6His-HA-PP1α.
Figure 1shows construct of the tetracycline-inducible plasmid used to express 6His-HA-PP1α.
If mRNA association with ribosomes is truly a measure of translation efficiency, one conclusion from these data is that in doxycycline-induced cells, 6His-HA-PP1α RNA is more efficiently translated than endogenous PP1α.
To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
A clear differential of expression of 6His-HA-PP1α (indicated at the left of the panel) was observed at 1.5 hours after addition of doxycycline, which became more pronounced at later time points.
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