Words similar to guanylyltransferase
Example sentences for: guanylyltransferase
How can you use “guanylyltransferase” in a sentence? Here are some example sentences to help you improve your vocabulary:
This is not a straw-man issue, given that S. cerevisiae encodes two homologous RNA triphosphatases (Cet1 and Cth1), of which only Cet1 is essential for capping and cell viability [ 8 15 ] . We use classical genetic approaches to show that the respective genes encoding RNA triphosphatase and RNA guanylyltransferase are essential in S. pombe.
Although the three capping reactions are universal in eukaryotes, there is a surprising diversity in the genetic organization of the capping enzymes as well as a complete divergence in the structure and catalytic mechanism of the RNA triphosphatase component in "lower" versus "higher" eukaryotic species [ 1 ] . Metazoans and plants have a two-component capping system consisting of a bifunctional triphosphatase-guanylyltransferase polypeptide and a separate methyltransferase polypeptide, whereas fungi contain a three-component system consisting of separate triphosphatase, guanylyltransferase, and methyltransferase gene products.
The triphosphatase (Cet1), guanylyltransferase (Ceg1), and methyltransferase (Abd1) components of the capping apparatus are essential for cell growth in the budding yeast S. cerevisiae [ 1 4 5 6 ] . Mutations of the RNA triphosphatase Cet1 that abrogate catalytic activity in vitro are lethal in vivo [ 7 8 9 ] ; thus, it is reasonable to think that pharmacological inhibition of Cet1 function in vivo would impede cell growth.
The cap is formed by three enzymatic reactions: the 5' triphosphate end of the nascent pre-mRNA is hydrolyzed to a diphosphate by RNA triphosphatase; the diphosphate end is capped with GMP by RNA guanylyltransferase; and the GpppN cap is methylated by RNA (guanine-7-) methyltransferase [ 1 ] .
S. pombeexpression vectors for RNA triphosphatase and guanylyltransferase
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