Words similar to guanine-nucleotide
Example sentences for: guanine-nucleotide
How can you use “guanine-nucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:
Thus, one might speculate that the novel 35 amino acid sequence in sα i2 would impair guanine-nucleotide binding and result in a unstable protein.
The A326S mutation of α i1 and A366S mutation of α s (cognate to the α i2 A327S used in this study) were shown to cause greatly decreased affinity for GDP [ 9 10 ] . Defective binding of GDP leads to more Gα in the empty state (no bound guanine-nucleotide), and this form of Gα is rapidly denatured in vitro , as shown for α s A366S and α i1 A326S [ 9 10 ] . Moreover, α s A366S was demonstrated to undergo rapid degradation (t 1/2 < 1 h) in stably transfected cells [ 9 ] . The same mutation in α t has also been shown to greatly decrease guanine-nucleotide binding [ 5 22 ] . Additional amino acids in the critical β6-α5 loop are important for maintaining Gα integrity [ 7 ] .
A recent study identified a number of α5 helix residues in α t that when changed to alanines increased rates of guanine-nucleotide exchange ( i.e., decreased affinity for guanine-nucleotides) [ 5 ] . Individual mutation of amino acids in α t , cognate to T330, N332, V333, F337 in α i2 (Figure 1), increased nucleotide exchange rates [ 5 ] . Two of these critical amino acids, N332 and V333 in α i2 , are in fact the first two amino acids that are replaced by the novel splicing in sα i2 (Figure 1).
The C-terminal 30 amino acids of Gα consist of the β6-α5 loop, followed by the α5 helix and finally several amino acids of flexible structure (Figure 1) [ 19 20 21 ] . The β6-α5 loop stabilizes the guanine ring of bound GDP or GTP, and mutations in this region affect guanine-nucleotide binding.
Although the α5 helix (Figure 1) does not directly contact the bound guanine-nucleotide [ 19 20 21 ] , it is clearly important for Gα structure and to maintain the proper orientation of the β6-α5 loop.
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