Words similar to guanidinium

Example sentences for: guanidinium

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Total RNA was extracted from individual conceptuses and pools of conceptuses of identical morphologies as previously described in our laboratory [ 8 ] . Briefly, conceptuses were denatured for 15 min on ice using 500 μl of denaturing solution (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7.0, 0.5% sarcosyl, 0.1 M 2-β-mercaptoethanol), 500 μl phenol, 70 μl 2 M sodium acetate (pH 4.0), and 140 μl chloroform/iso-amyl-alcohol (49:1 fresh dilution).
CAP retained on top of the filter was quantitated as a complex with ruthenium red [ 21 ] . The retentate on the 100,000 M w cut-off filter was dissolved in 1 ml of 2 M guanidinium hydrochloride and CAP released from the CAP-gp120 complex and retained on the 50,000 M w cut-off filter was quantitated by the same method.
Briefly, total RNA was isolated from lung tissue by standard guanidinium isothiocyanate/cesium chloride gradients [ 43].
. Deionized formamide Distilled Phenol and guanidinium isothiocyanate were purchased from Fluka (Milwaukee, Wl).