Example sentences for: guanidine

How can you use “guanidine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA was isolated using guanidine thiocyanate extraction and 15 μg of the total RNA was analyzed by Northern hybridization as described previously [ 16 ] . cDNA probes used in hybridization were human fibronectin [ 33 ] , α1(I) collagen [ 34 ] , or cytokeratin 14 cDNA (Hung Tseng, unpublished results).

  • Inclusion bodies containing the protein were prepared and then solubized in 6 M guanidine.

  • The factor V Leiden mutation is located in exon 10, 11 nucleotides 5' of the start of intron 10 at nucleotide 1691, where an adenosine replaces guanidine [ 15].

  • Total RNA was purified by homogenization with guanidine isothiocyanate and phenol/chloroform extraction [ 28 ] . All procedures was performed at neutral pH.

  • The purified ButaIT was reduced and carboxymethylated by incubating in 6 M guanidine hydrochloride, 0.1 M Tris-HCl (pH 8.3), 1 mM EDTA and 20 mM dithiothreitol for 1 hr at 37°C.


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