Words similar to gstπ

Example sentences for: gstπ

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Two sets of primers were used in all reactions to yield the amplification of an endogenous control gene (β-actin, 383 bases) and the specific target genes of interest [157 bases of MDR1 (a gift from Jian Lin, Cancer Center/Institute of Cancer Research, Golumbia University, College of Physicians and Surgeons, New York, USA), 270 bases of GSTπ, 203 bases of MRP and 139 bases of TopoIIα independently (Table 1) [ 15]].
ADR = adriamycin; DRO = droloxifen; GSTπ = glutathione S-transferase Pi; MDR = multidrug resistance; MG = megestrol; MRP = MDR-related protein; MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H -tetrazolium bromide; NOM = nomegestrol; PBS = phosphate-buffered saline; P-gp = P-glycoprotein; RT-PCR = reverse transcriptase polymerase chain reaction; TAM = tamoxifen; TopoIIα = topoisomerase IIα; VRP = verapamil.
MCF7/WT cells expressed a high level of TopoIIα only: P-gp and GSTπ protein expression were undetectable.
In brief, after three washes with phosphate-buffered saline (PBS), fixed cells in each smear were layered with the primary antibody against P-gp (1:20 dilution), GSTπ (1:25 dilution) and TopoIIα (1:50 dilution) and incubated overnight (a minimum of 18 h).
Monoclonal antibody against P-gp (JSB-1) was purchased from Boehringer Mannheim Biochemica; monoclonal antibodies against GSTπ and TopoIIα were purchased from Dako Corporation and Neomarker, respectively.