Words similar to gstπ
Example sentences for: gstπ
How can you use “gstπ” in a sentence? Here are some example sentences to help you improve your vocabulary:
After treatment with 5 μM NOM, the MDR1 and GSTπ expression levels of MCF7/ADR cells were modulated in a time-dependent manner.
In brief, after three washes with phosphate-buffered saline (PBS), fixed cells in each smear were layered with the primary antibody against P-gp (1:20 dilution), GSTπ (1:25 dilution) and TopoIIα (1:50 dilution) and incubated overnight (a minimum of 18 h).
We found that NOM markedly decreased the level of expression of GSTπ mRNA and protein with a time course similar to that of MDR1/P-gp.
MCF7/WT cells expressed a high level of TopoIIα only: P-gp and GSTπ protein expression were undetectable.
For MDR1, GSTπ and TopoIIα genes, after an initial denaturation at 94°C for 1.5 min, 30 cycles of PCR amplification were performed, each cycle consisting of a denaturing step of 94°C for 1.5 min, annealing at 54°C for 1 min, and extension at 72°C for 3 min, followed by a final step at 72°C for 10 min.