Words similar to gstπ

Example sentences for: gstπ

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After treatment with 5 μM NOM, the MDR1 and GSTπ expression levels of MCF7/ADR cells were modulated in a time-dependent manner.
Two sets of primers were used in all reactions to yield the amplification of an endogenous control gene (β-actin, 383 bases) and the specific target genes of interest [157 bases of MDR1 (a gift from Jian Lin, Cancer Center/Institute of Cancer Research, Golumbia University, College of Physicians and Surgeons, New York, USA), 270 bases of GSTπ, 203 bases of MRP and 139 bases of TopoIIα independently (Table 1) [ 15]].
For MDR1, GSTπ and TopoIIα genes, after an initial denaturation at 94°C for 1.5 min, 30 cycles of PCR amplification were performed, each cycle consisting of a denaturing step of 94°C for 1.5 min, annealing at 54°C for 1 min, and extension at 72°C for 3 min, followed by a final step at 72°C for 10 min.
MCF7/WT cells expressed a high level of TopoIIα only: P-gp and GSTπ protein expression were undetectable.
In brief, after three washes with phosphate-buffered saline (PBS), fixed cells in each smear were layered with the primary antibody against P-gp (1:20 dilution), GSTπ (1:25 dilution) and TopoIIα (1:50 dilution) and incubated overnight (a minimum of 18 h).