Words similar to gstp
Example sentences for: gstp
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The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
In addition, GSTP1-1 acts as a negative regulator of JNK primarily through direct protein:protein interactions [ 9 10 ] . These data suggest that GSTP1-1 has a role in regulation of kinase activities and cell proliferation [ 8 11 12 13 ] . Because of this available background information, the HL60 wild type and TLK286 resistant cell lines were chosen as model systems for analysis by ADGE microarray.
6%; APC , 9.8%; RARB , 7.6%; CDH1 , 7.6%; FHIT , 6.5%, MLH1 , 4.3%; TIMP3 , 3.3%; GSTP1 , 1.1%; and NME2 , 1.1% (Fig.
Of these, 15 genes ( MGMT , RASSF1A , APC , RARB , CDH1 , MLH1 , TIMP3 , GSTP1 , DAPK , CDKN2A , p14 ARF , BRCA1 , FHIT , TP73 , and HIC1 ) had previously been shown to be commonly methylated in various solid tumor types [ 4 ] . Six additional genes ( RB1 , NME1 , NME2 , BTG1 , NEDD1 , and APAF1 ) were studied because of their possible involvement in genetic alterations in GCT as indicated by LOH studies [ 8 9 10 ] . The RB1 gene at 13q14.
In addition, expression of GSTP1-1 was reduced in the HL60/TLK286 cell line, which was consistent with a previous study [ 7 ] . Quantitative RT-PCR confirmed the results of ADGE microarray for eleven of thirteen genes.
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