Example sentences for: gst-pulldown

How can you use “gst-pulldown” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The GST-pulldown assay was done as previously described [ 22].

  • C-terminal deletions of either protein eliminated binding to TOP1 (C-terminal truncation of 112 aa of BTBD1 or 33 aa of BTBD2 in the two-hybrid assay; or a C-terminal truncation of 40 aa of BTBD2 in GST-pulldown).

  • The GST-pulldown assay confirmed the positive interactions seen in the two-hybrid deletion mapping (Fig.

  • Therefore, we used a GST-pulldown assay to determine if the proteins could interact in vitro . p35-C/EBPβ was expressed as a chimeric GST protein and immobilized on glutathione-agarose beads.

  • Taken together, the results of the two-hybrid and GST-pulldown assays narrow the interacting region of BTBD1 and BTBD2 to roughly the C-terminal half of each protein with critical residues within the last 50 residues.


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