Words similar to gst-pulldown
Example sentences for: gst-pulldown
How can you use “gst-pulldown” in a sentence? Here are some example sentences to help you improve your vocabulary:
The GST-pulldown assay was done as previously described [ 22].
C-terminal deletions of either protein eliminated binding to TOP1 (C-terminal truncation of 112 aa of BTBD1 or 33 aa of BTBD2 in the two-hybrid assay; or a C-terminal truncation of 40 aa of BTBD2 in GST-pulldown).
The GST-pulldown assay confirmed the positive interactions seen in the two-hybrid deletion mapping (Fig.
Therefore, we used a GST-pulldown assay to determine if the proteins could interact in vitro . p35-C/EBPβ was expressed as a chimeric GST protein and immobilized on glutathione-agarose beads.
Taken together, the results of the two-hybrid and GST-pulldown assays narrow the interacting region of BTBD1 and BTBD2 to roughly the C-terminal half of each protein with critical residues within the last 50 residues.