Words similar to gst-p
Example sentences for: gst-p
How can you use “gst-p” in a sentence? Here are some example sentences to help you improve your vocabulary:
Glutathione agarose beads containing GST-p35-C/EBPβ or GST alone were incubated with in vitro -translated Elk-1 mutants labeled with [ 35S] methionine.
GST-p35-C/EBPβ, GST-p20-C/EBPβ, or GST alone was produced in BL21 E. Coli by 80 mM isopropyl-β-D-thiogalactopyranoside (IPTG) induction overnight at 37°C.
6A) and tested these constructs in a pulldown assay with GST-p35-C/EBPβ.
However, it is clear that the binding of Elk-1 is substantially increased when the GST-p35-C/EBPβ fusion protein is present on the beads.
6B, the C-terminal Elk-1 mutants, Elk-1(1-209) and Elk-1(1-140), bound to the GST-p35-C/EBPβ beads to the same extent as wild-type Elk-1 (compare lane 3 of top 3 panels).