Words similar to gst-fusion
Example sentences for: gst-fusion
How can you use “gst-fusion” in a sentence? Here are some example sentences to help you improve your vocabulary:
To construct the GST-fusion protein expression vectors, the BTBD inserts from the prey vector pPC86 were amplified using primers complementary to the BTBD coding regions with additional Sma I and EcoR I restriction sites for insertion into these sites in pGEX-2TK (Pharmacia).
For the GST-pull-down assay 5 μg of GST or the GST-fusion proteins were mixed with 150 μl HeLa cell nuclear extracts (5 × 10 6cells/ml) and incubated for 2 hr at 4°C (0.
Recombinant Ppt1p expressed as a GST-fusion protein, then affinity purified and released from GST by thrombin cleavage yielded a single band of the expected molecular weight, 58 kDa (Fig.