Words similar to gst-cdc

Example sentences for: gst-cdc

How can you use “gst-cdc” in a sentence? Here are some example sentences to help you improve your vocabulary:

Net1N includes the N-terminal 341 amino acids of Net1 and is sufficient to bind and inhibit Cdc14 [ 11 ] . When GST-Cdc14/His6-T7-Net1N complex anchored to anti-T7 beads was treated with ATP plus increasing amounts of recombinant Cdc5 purified from insect cells, both Cdc14 and Net1N were phosphorylated as indicated by their increased apparent molecular weight.
GST-Cdc6 eluted from the beads following high salt wash failed to show any DNA binding activity (Fig.
p35 was purified as high salt (300 mM NaCl) eluate from the GST-Cdc6 beads.
To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.
4 mg/ml; GST-Cdc14~0.