Words similar to gst-cdc

Example sentences for: gst-cdc

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GST-T7-Cdc14 (RDB 872) and GST-Cdc14 (RDB 1183) were expressed in E. coli BL21(DE3) pLysS cells, and purified on glutathione-agarose beads (Sigma) as described previously [ 33 ] . His6-T7-Net1(1-341) (Net1N, RDB1153) [ 11 ] was expressed in E. coli BL21(DE3) pLysS cells, and purified on Ni-NTA agarose beads (Qiagen) as described previously for p28 Kix1 [ 34 ] . Purification of His6-Plx1, His6-Plx1(N172), and His6-HA-Cdc5 from baculovirus-infected insect cells followed an earlier protocol [ 24 ] . The concentrations of purified proteins were in the ranges of: Net1N~0.
4 mg/ml; GST-Cdc14~0.
p35 was purified as high salt (300 mM NaCl) eluate from the GST-Cdc6 beads.
GST-Cdc6 eluted from the beads following high salt wash failed to show any DNA binding activity (Fig.
NM participated in insect cell culture and helped in making and amplification of baculovirus expressing GST-Cdc6 and GST.