Words similar to gst-cdc

Example sentences for: gst-cdc

How can you use “gst-cdc” in a sentence? Here are some example sentences to help you improve your vocabulary:

GST-Cdc6 eluted from the beads following high salt wash failed to show any DNA binding activity (Fig.
4 mg/ml; GST-Cdc14~0.
GST-T7-Cdc14 (RDB 872) and GST-Cdc14 (RDB 1183) were expressed in E. coli BL21(DE3) pLysS cells, and purified on glutathione-agarose beads (Sigma) as described previously [ 33 ] . His6-T7-Net1(1-341) (Net1N, RDB1153) [ 11 ] was expressed in E. coli BL21(DE3) pLysS cells, and purified on Ni-NTA agarose beads (Qiagen) as described previously for p28 Kix1 [ 34 ] . Purification of His6-Plx1, His6-Plx1(N172), and His6-HA-Cdc5 from baculovirus-infected insect cells followed an earlier protocol [ 24 ] . The concentrations of purified proteins were in the ranges of: Net1N~0.
To test if Cdc5 could dislodge Cdc14 from Net1, we devised a simplified in vitro release assay using GST-Cdc14 and His6-T7-Net1N expressed and purified from bacteria.
Net1N includes the N-terminal 341 amino acids of Net1 and is sufficient to bind and inhibit Cdc14 [ 11 ] . When GST-Cdc14/His6-T7-Net1N complex anchored to anti-T7 beads was treated with ATP plus increasing amounts of recombinant Cdc5 purified from insect cells, both Cdc14 and Net1N were phosphorylated as indicated by their increased apparent molecular weight.