Words similar to gst-cdc

Example sentences for: gst-cdc

How can you use “gst-cdc” in a sentence? Here are some example sentences to help you improve your vocabulary:

To test if Cdc5 could dislodge Cdc14 from Net1, we devised a simplified in vitro release assay using GST-Cdc14 and His6-T7-Net1N expressed and purified from bacteria.
GST-Cdc6 eluted from the beads following high salt wash failed to show any DNA binding activity (Fig.
NM participated in insect cell culture and helped in making and amplification of baculovirus expressing GST-Cdc6 and GST.
Net1N includes the N-terminal 341 amino acids of Net1 and is sufficient to bind and inhibit Cdc14 [ 11 ] . When GST-Cdc14/His6-T7-Net1N complex anchored to anti-T7 beads was treated with ATP plus increasing amounts of recombinant Cdc5 purified from insect cells, both Cdc14 and Net1N were phosphorylated as indicated by their increased apparent molecular weight.
To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.