Words similar to gst

Example sentences for: gst

How can you use “gst” in a sentence? Here are some example sentences to help you improve your vocabulary:

An RGS3-GST fusion protein was incubated with an 35S-Met-Gα-protein, and the amount of labeled Gα-protein was determined using glutathione-coupled beads to "pull down" the RGS3-GST/ 35S-Gα-protein complex that was then separated and quantified by SDS/PAGE (Fig.
The dialyzed sample was then bound to GST beads (Sigma) and washed three times with buffer H*/0.
Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.
It is important to note that majority of the p35 bound to GST or GST fusion proteins are released mostly at high salt concentration (300 mM) allowing us to get rid of most of the impurities by stringent washing of the GST beads with buffer containing 250 mM NaCl.
The fusion protein was then cleaved with thrombin (Calbiochem, San Diego, CA) to yield the actin-binding domain of α-actinin and the GST carrier.