Example sentences for: gst

How can you use “gst” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • aa, amino acid; Ab, antibody; bp, base pair; CaPO 4 , calcium phosphate; C/EBPβ, CCAAT-enhancer binding protein-beta; CAT, chloramphenicol acetyl transferase; DMEM, Dulbecco's Modified Eagle Medium; GST, glutathione-S-transferase; MAPK, mitogen activated protein kinase; PBS, phosphate buffered saline; PMSF, phenylmethylsulfonyl fluoride; SRE, serum response element; SRF, serum response factor; TCF, ternary complex factor.

  • 4 with 0.1% Triton X-100, insoluble material removed by centrifugation for 10 minutes at 14,000 rpm and soluble GST fusion proteins purified with GST-Sepharose beads (Pharmacia).

  • Sf9 insect cells were infected with baculovirus expressing GST alone.

  • Therefore, it can be concluded that p35 interacts with GST and high salt concentration is required to disrupt this interaction.

  • IVT proteins were incubated with the GST fusion proteins on beads for 1 hour at 4°C in 500 μL of 40 mM HEPES pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM EDTA, 1% Triton X100, 1 mM ATP, 1 μM microcystin, 14 mM b-mercaptoethanol.


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