Words similar to gst

Example sentences for: gst

How can you use “gst” in a sentence? Here are some example sentences to help you improve your vocabulary:

Induction of GST-PNUTS fusion protein synthesis and isolation by affinity chromatography using glutathione-Sepharose beads (Pharmacia) was performed as described previously [ 32 ] . Induced LLWO2F cell extracts were then incubated for 1 hour at 4°C with recombinant GST or GST-PNUTS fusion protein bound to glutathione-Sepharose.
IVT proteins were incubated with the GST fusion proteins on beads for 1 hour at 4°C in 500 μL of 40 mM HEPES pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM EDTA, 1% Triton X100, 1 mM ATP, 1 μM microcystin, 14 mM b-mercaptoethanol.
PH, pleckstrin homology; PI3K, phosphoinositide 3-Kinase; SH2, Src homology-2, SH3, Src homology-3; XLA, X-linked agammaglobulinemia; xid ; x-linked immunodeficiency; TCR, T cell receptor; sIgM, surface immunoglobulin M; (GAP), GTPase-Activating Protein; GST, Glutathione S-Transferase; SDS, Sodium Dodecyl Sulfate; PBS, phosphate buffered saline; NP40, Nonidet P-40; PAGE, polyacrylamide gel electrophoresis.
PN Performed transfection, mutagenesis and GST pulldown experiments under guidance of PW.
For Western blots, we used 1:2000 9E10, 1:2500 α-Sir2, 1:1000 rabbit α-Cdc14 (crude serum), 1:6000 α-GST, and 1:5000 α-T7 (Novagen).