Words similar to gst
Example sentences for: gst
How can you use “gst” in a sentence? Here are some example sentences to help you improve your vocabulary:
aa, amino acid; Ab, antibody; bp, base pair; CaPO 4 , calcium phosphate; C/EBPβ, CCAAT-enhancer binding protein-beta; CAT, chloramphenicol acetyl transferase; DMEM, Dulbecco's Modified Eagle Medium; GST, glutathione-S-transferase; MAPK, mitogen activated protein kinase; PBS, phosphate buffered saline; PMSF, phenylmethylsulfonyl fluoride; SRE, serum response element; SRF, serum response factor; TCF, ternary complex factor.
4 with 0.1% Triton X-100, insoluble material removed by centrifugation for 10 minutes at 14,000 rpm and soluble GST fusion proteins purified with GST-Sepharose beads (Pharmacia).
Sf9 insect cells were infected with baculovirus expressing GST alone.
Therefore, it can be concluded that p35 interacts with GST and high salt concentration is required to disrupt this interaction.
IVT proteins were incubated with the GST fusion proteins on beads for 1 hour at 4°C in 500 μL of 40 mM HEPES pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM EDTA, 1% Triton X100, 1 mM ATP, 1 μM microcystin, 14 mM b-mercaptoethanol.