Words similar to gst

Example sentences for: gst

How can you use “gst” in a sentence? Here are some example sentences to help you improve your vocabulary:

It was latter found that the p35 has an intrinsic affinity to GST.
p35 has an intrinsic affinity to GST moieties.
A fragment containing the PPT1 ORF was excised from the pBII-PPT1 using Eco RI and Xho I restriction enzymes and was subcloned downstream of the GST coding region into the Eco RI and Sal I sites of the bacterial expression vector pET-21a GST [ 37 ] . To construct the pET-GST-PPT1 (1-504) plasmid a 453 bp fragment was amplified from pET-GST-PPT1 by PCR using a 3' primer containing a Xho I restriction site and a stop codon designed to truncate the PPT1 gene product at Met504 (5'-gatcctcgagTTAcattggttttatatctggg) and a 5' primer (5'-gtaatgcatggtggttta) encompassing the Nsi I restriction site of PPT1 . The PCR product was cloned into the Nsi I and Xho I restriction sites of pET-GST-PPT1 and sequenced.
Reactions were terminated by the addition of SDS sample buffer and p53-GST phosphorylation detected by SDS-PAGE and auotradiography.
Since there was no significant phosphorylation of p53-GST by GSK3α (fig 1), this indicates that phosphorylation of p53 by DNA-PK, in vitro, creates a site for GSK3β to phosphorylate p53.