Words similar to gst-btbd
Example sentences for: gst-btbd
How can you use “gst-btbd” in a sentence? Here are some example sentences to help you improve your vocabulary:
Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.
E. coli containing plasmids encoding GST-BTBD1 or GST-BTBD2 were grown to an O.D.
To test the effect of BTBD2 on the supercoil relaxation activity of TOP1, HeLa cell nuclear extracts containing TOP1 were incubated with GST or GST-BTBD2 (aa 86-525).
GST-BTBD2 addition produced a slight inhibition of TOP1 cleavage activity relative to GST alone (Fig.
GST-BTBD2 slightly inhibited the supercoil relaxation activity of TOP1, increasing the time required to completely relax the supercoiled DNA by about two-fold as compared with GST alone (Fig.