Words similar to gst-btbd

Example sentences for: gst-btbd

How can you use “gst-btbd” in a sentence? Here are some example sentences to help you improve your vocabulary:

GST-BTBD2 slightly inhibited the supercoil relaxation activity of TOP1, increasing the time required to completely relax the supercoiled DNA by about two-fold as compared with GST alone (Fig.
The region of TOP1 sufficient for binding BTBD2 was narrowed to residues 215 - 329 in the "cap" region (core subdomain II) [ 31 ] . Catalytic activities of TOP1 were affected slightly or not at all by co-incubation with GST-BTBD2 suggesting that the interaction does not regulate enzyme activity directly.
To test the effect of BTBD2 on the supercoil relaxation activity of TOP1, HeLa cell nuclear extracts containing TOP1 were incubated with GST or GST-BTBD2 (aa 86-525).
GST-BTBD2 addition produced a slight inhibition of TOP1 cleavage activity relative to GST alone (Fig.
HeLa nuclear extract (5 ng) in reaction buffer (100 mM Tris.HCl, pH 7.5, 50 mM KCl, 5 mM MgCl 2 , 0.1 mM EDTA, 15 μg/ml BSA) was incubated with 5 μg of purified GST or GST-BTBD2 on ice for 15 min before the addition of 0.25 μg plasmid and incubation at 30°C for 0-24 min.