Words similar to gst-btbd

Example sentences for: gst-btbd

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GST-BTBD2 addition produced a slight inhibition of TOP1 cleavage activity relative to GST alone (Fig.
HeLa nuclear extract (5 ng) in reaction buffer (100 mM Tris.HCl, pH 7.5, 50 mM KCl, 5 mM MgCl 2 , 0.1 mM EDTA, 15 μg/ml BSA) was incubated with 5 μg of purified GST or GST-BTBD2 on ice for 15 min before the addition of 0.25 μg plasmid and incubation at 30°C for 0-24 min.
To test the effect of BTBD2 on the supercoil relaxation activity of TOP1, HeLa cell nuclear extracts containing TOP1 were incubated with GST or GST-BTBD2 (aa 86-525).
The region of TOP1 sufficient for binding BTBD2 was narrowed to residues 215 - 329 in the "cap" region (core subdomain II) [ 31 ] . Catalytic activities of TOP1 were affected slightly or not at all by co-incubation with GST-BTBD2 suggesting that the interaction does not regulate enzyme activity directly.
GST-BTBD2 slightly inhibited the supercoil relaxation activity of TOP1, increasing the time required to completely relax the supercoiled DNA by about two-fold as compared with GST alone (Fig.