Example sentences for: gnrh-a

How can you use “gnrh-a” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Bound and free [ 125I]GnRH-A were separated by filtration.

  • cDNA representing the rat GnRH receptor was cloned into pcDNA3 as described and validated previously [ 22 23 ] . cDNAs representing RGS3 and RGS3T (RGS3 amino acids 314-519) were cloned into the eukaryotic expression vector, rcCMV, as described [ 24 ] . COS-1 cells cultured as detailed before [ 23 ] were co-transfected with GnRH receptor cDNA and RGS3 or RGS3T cDNA using Lipofectamine (Life Technologies, Gaithersburg, MD) [ 23 ] , and 72 h later, treated with 10 -7M GnRH-A for 0, 10, 20, or 30 sec before being collected for IP 3 measurements [ 22 23 ] .

  • Then, the cells were stimulated with GnRH-A (10 -7M for one hr) in the presence of 5 mM LiCI.

  • 1% bovine serum albumin (BSA) and subjected to x-gal staining for determination of the fraction of cells infected by the virus [ 31 ] , or to stimulation by GnRH-A for measurement of LH release.

  • Pituitary cells that had been re-trypsinized 72 h after infection and then replated (100,000 cells/well in a 24-well poly-L-lysine coated plate) for a 2 h preincubation were subsequently treated with a GnRH agonist (1 ml of 10 -7M D-Ala 6- des Gly 10- GnRH ethylamide; GnRH-A, Sigma, St. Louis, MO) or a calcium ionophore (A23187, Sigma) for 3 h as described by Conn et al.


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