Words similar to gnrh-a
Example sentences for: gnrh-a
How can you use “gnrh-a” in a sentence? Here are some example sentences to help you improve your vocabulary:
Then, the cells were stimulated with GnRH-A (10 -7M for one hr) in the presence of 5 mM LiCI.
1% bovine serum albumin (BSA) and subjected to x-gal staining for determination of the fraction of cells infected by the virus [ 31 ] , or to stimulation by GnRH-A for measurement of LH release.
cDNA representing the rat GnRH receptor was cloned into pcDNA3 as described and validated previously [ 22 23 ] . cDNAs representing RGS3 and RGS3T (RGS3 amino acids 314-519) were cloned into the eukaryotic expression vector, rcCMV, as described [ 24 ] . COS-1 cells cultured as detailed before [ 23 ] were co-transfected with GnRH receptor cDNA and RGS3 or RGS3T cDNA using Lipofectamine (Life Technologies, Gaithersburg, MD) [ 23 ] , and 72 h later, treated with 10 -7M GnRH-A for 0, 10, 20, or 30 sec before being collected for IP 3 measurements [ 22 23 ] .
Bound and free [ 125I]GnRH-A were separated by filtration.
The GnRH RRA on the membranes was performed as described previously [ 22 23 ] . Cell membranes and 1 × 10 5cpm [ 125I]GnRH-A were incubated at 0°C for 90 min.