Words similar to gly-

Example sentences for: gly-

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Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.
Apart from the curious substitution of 15 of 16 nucleotide-proximal residues in Asw, the most dissimilar region of Asw consists of a five amino-acid insertion between strands β1 and β2 and substitution of Gly-Ala-Pro (Asw) for Asp-Glu-Ser (Hint) at the amino-terminal end of helix α2.
Linear increase of absorbance at 405 nm were recorded after adding S-2238 (final concentration 0.25 mM) for thrombin, or N-p-Tosyl-Gly-Pro-Lys-p-Nitroanilide (final concentration 0.5 mM) for plasmin.
After two rounds of routing, synthesis, and selection, followed by DNA sequencing, the remaining oligonucleotides predominantly encoded the Tyr-Gly-Gly-Phe-Leu sequence or close variants thereof.
A chromogenic plasminogen activator substrate (Boc-Val-Gly-Arg-βNA.AcOH) was purchased from Calbiochem.