Words similar to glyb
Example sentences for: glyb
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The final entry in table 1is for ThDP uptake by mitochondria isolated from glyB cells.
There is a high degree of amino acid similarity among folate transporters and the thiamine transporter of the plasma membrane [ 7 8 9 10 ] . Recently, it was found that in murine cells there can be a substantial efflux of ThDP mediated by the reduced folate carrier protein [ 22 ] . GlyB cells are a Chinese hamster ovary cell line derivative that are deficient in the transport of folates into mitochondria, and the responsible mitochondrial transporter has recently been identified and its gene cloned [ 23 ] . We wondered if the mitochondrial folate transporter was responsible for uptake of ThDP into mitochondria, making analogies to the ability of the plasma membrane folate transporter being able to transport ThDP.
All cell types were growth at 37°C in the presence of 10 μM thiamine in RPMI 1640 medium supplemented with 10% heat-inactive fetal calf serum, 2 mM L-glutamine and 1 g/L penicillin/streptomycin, except glyB cells which were grown in MEM medium.
However, this is not the case as glyB cells that lack the mitochondrial folate transporter were found to take up ThDP with high affinity kinetics similar to that of mitochondria of human cells.
Normal lymphoblasts, TRMA lymphoblasts and fibroblasts cell lines were obtained and have been characterized as described [ 6 ] . The human neuroblastoma cells were an SY-SY5Y cell line, a thrice-cloned subline of SK-N-SH [ 24 ] . GlyB cells, a Chinese hamster ovary K1 subline, are deficient in the transport of folate into mitochondria [ 23 ] and were a kind gift from L. Chasin (Columbia University).
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