Example sentences for: glutamine

How can you use “glutamine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The deduced product of a third M. incognita gene (MI00426) has striking sequence similarity to glutamine synthetase (GS).

  • The resulting pMIRB-HA-NPM3 plasmids (both sense and antisense orientations) were transiently transfected into NIH 3T3 cells, using transfection conditions with Lipofectamine and OptiMEM serum-free medium (Gibco-BRL, Bethesda, MD) as described [ 49 ] . Following a six-hour incubation of the DNA-Lipofectamine complexes in OptiMEM, the cells were washed and incubated in 10-cm dishes with their usual growth media (DMEM with 10% v/v fetal calf serum, 2 mM L-glutamine, 100 Units/ml of Penicillin G and 100 μg/ml Streptomycin) for 48-72 hours at 37°C in humidified 5% CO 2 incubators.

  • They were routinely grown in a monolayer (30%-50% confluence) on a glass cover slide at 37°C in DMEM (Dulbecco's Modified Eagle Medium), supplemented with 1% L -glutamine, 1% HEPES [4-(2-hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)], 0.5% gentamicin, 1% penicillin/streptomycin and 10% Fetal Bovine Serum (Gibco, NY).

  • Tobramycin, L-glutamine and 0.25% porcine trypsin - 0.53 mM EDTA were from Abbott Laboratories, BioWittaker and Gibco correspondingly.

  • SUDHL-5, SUDHL-6, WSU-1, Jurkat, MCF-7, PC3, BJAB, T cells, monocytes and PBMCs were maintained in RPMI 1640 medium with L-glutamine, HEPES, penicillin, streptomycin and 10% fetal bovine serum.


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