Words similar to glutamine
Example sentences for: glutamine
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All cultures were grown in serum-free Neurobasal ™medium (2 ml per dish) supplemented with B27 (Gibco BRL, Grand Island, NY; 50:1 v/v), 0.5 mM L-glutamine (Sigma) and 100 units/ml penicillin-streptomycin (Gibco; Catalog No.
1 sequence, include in sequential order: 1) A DxxTTxxDxxWR motif immediately preceding M1, 2) a highly conserved tryptophan in M1, 3) an insertion in the M1-P loop that contains a CVxx (V or I) motif, 4) the absence of an aromatic residue at the first 'aromatic cuff position [ 2 ] in the P segment helix, 5) a glutamine near the end of the P helix, 6) the absence of an aspartate at the end of the P segment signature sequence (TVGYGD) and a RxxTxxCP motif in the P-M2 loop that is two residues longer than in most other K +channels, 7) a glutamine in the first part of S6, and 8) a hydrophilic residue (Asn, Asp, or Glu) near the mid region of S6 that when negatively charged is involved in blockade of some Kir's by Mg 2+ [ 9 ] . Fig.
On the basis of both amino-acid sequence similarity (Table 3) and a Pfam [ 36 ] HMM search (e-value 4.3e -24), it is clear that the RKN glutamine synthetase is a GSI homolog, implying a prokaryotic origin.
The resulting pMIRB-HA-NPM3 plasmids (both sense and antisense orientations) were transiently transfected into NIH 3T3 cells, using transfection conditions with Lipofectamine and OptiMEM serum-free medium (Gibco-BRL, Bethesda, MD) as described [ 49 ] . Following a six-hour incubation of the DNA-Lipofectamine complexes in OptiMEM, the cells were washed and incubated in 10-cm dishes with their usual growth media (DMEM with 10% v/v fetal calf serum, 2 mM L-glutamine, 100 Units/ml of Penicillin G and 100 μg/ml Streptomycin) for 48-72 hours at 37°C in humidified 5% CO 2 incubators.
The amino acids Methionine, Proline, Tryptophan and Glutamine occur predominantly in the rigid tripeptides and not as much in the other two categories, suggesting that Methionine, Proline, Tryptophan and Glutamine can cause structural rigidity in a tripeptide.
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