Example sentences for: gfp-blm

How can you use “gfp-blm” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Highly purified GFP-BLM eluted in Buffer B + 200 mM imidazole and was stored at -70°C.

  • The GFP-BLM fusion protein has helicase activity in vitro and functions to reduce the SCE frequency in BS cells.

  • Tet concentrations from 0.25-2 μg/ml produced levels of stable GFP-BLM expression in this SV40-transformed BS cell line similar to the amount found in the control cell line.

  • Pooled Macroprep Q fractions containing GFP-BLM were loaded onto a 5 ml HiTrap metal chelate column (Amersham Pharmacia) charged with NiS0 4 equilibrated in Buffer B. The metal chelate resin was washed with successive 10 column volumes of Buffer B + 10mm imidazole, Buffer B + 50 mM imidazole, and Buffer B + l00 mM imidazole.

  • The BS cell line used here lacks BLM [ 10] and all cell lines obtained from transfection of the normal GFP-BLM gene had a background level of constitutive expression leading to a partially corrected SCE frequency relative to the parental line (40 vs. 58) [ 10].


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