Words similar to gfp-cop

Example sentences for: gfp-cop

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Most GFP-COP1 lines and the GUS-COP1 line L4 have been described [ 27 41 42 ] . Unsilenced GUS-COP1 and GFP-COP1 transgenes complement the cop1 mutation [ 42 43 ] . The C11 and C71 loci contain at least three and two linked T-DNAs, respectively, as seen by Southern blotting (not shown).
In our transgene-based system, the silencer loci GFP-COP1 C73 and C97 underwent an apparently spontaneous transition in their silencing behavior between the T2 and T3 generations (Fig.
As shown in figure 5Aand 5B, GFP-ΔN70 (amino acid 71-733), which deleted the vertebrate specific N-terminal extension, also exhibited three general localization patterns in transfected cells, mostly cytoplasmic, in both compartments, or mostly nuclear, similar to full-length GFP-COP1 (Fig 3C).
The trans-silencers as well as their targets reside in single-copy, gene-rich regions that are only sparsely populated with repetitive or transposable elements, features that have been shown to mediate epigenetic activity in other cases [ 18 31 32 ] . Therefore trans-silencing of GFP-COP1 is probably not mediated by transgene flanking sequences [ 27 ] . In contrast, as with other genes cited above, trans-silencing ability is correlated with transgene locus structure, given that the C73 and C97 trans-silencers contain multiple T-DNAs while their targets, E82 and L91, are essentially dimeric and monomeric, respectively.
Individual F1 dihybrid plants were inspected for GFP-COP1 and COP1 endogene silencing phenotypes.