Words similar to gentamycin
Example sentences for: gentamycin
How can you use “gentamycin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Complete methods for the stable transfection of HEK-293 cells to express the wild type receptor can be found in a previous report [ 13 ] . Both cell lines were seeded in 6-well dishes at an initial cell density of 1 × 10 6cells/well and were maintained in Dulbecco's modified Eagle's medium (DMEM; GibcoBRL) supplemented with 2 mM L-glutamine, 5% fetal bovine serum (FBS) (Gemini Bio-Products, Calabasas, CA), and 100 μg/ml gentamycin sulfate (Gemini Bio-Products, Calabasas, CA).
The in vitro -adapted BCL 1 .3B3 cell line was previously selected from the BCL 1 mouse B cell lymphoma for growth in cell culture [ 60 ] . BCL 1 .3B3 cells were grown in RPMI supplemented with 25 μg/ml gentamycin, 2 mM L-glutamine, 100 U/ml penicillin, 100 U/ml streptomycin, non-essential amino acids (0.
P. falciparum parasite cells (W2 strain) were cultured as described [ 54] with slight modifications: 2% suspension of purified human red blood cells in RPMI1640 media supplemented with 0.25% AlbumaxI (GIBCO/Invitrogen, San Diego, CA), 2 g/l sodium bicarbonate, 0.1 mM hypoxanthine, 25 mM HEPES pH 7.4, and 50 μg/I gentamycin.
Placental tissue was digested and trophoblast cells isolated and cultured as described previously [ 15 36 ] . Briefly, the cells collected after digestion were pooled, washed twice in DMEM containing 25 mM HEPES and 4500 mg/L glucose (DMEM-HG), diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.
After 1 h, gentamycin was added at 100 μg/ml to kill any remaining extracellular bacteria.
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