Words similar to gentamicin
Example sentences for: gentamicin
How can you use “gentamicin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were maintained in Medium 199/MCDB 105 (1:1) (Sigma, St. Louis, MO) with 5% fetal bovine serum (GibcoBRL, Rockville, MD) and 10 ug/ml gentamicin (GibcoBRL) in 5% CO 2 /95% air at 37°C as described previously [ 31 40 ] .
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
RA SCL, osteoarthritis (OA) SCL, and skin fibroblasts (1 × 10 5) were seeded in six-well culture plates (Costar) with DMEM containing 10% FBS, 200 U/ml penicillinG, 10 μg/ml gentamicin and 0.3 mg/ml L-glutamine, and cultured for 3 days.
For selection of E. coli bacteria, drugs were incorporated into the medium at the following concentrations: hygromycin (Invitrogen, Carlsbad, CA), 200 μg/m; kanamycin (Sigma), 25 μg/ml; zeocin (Invitrogen), 25 μg/ml; and gentamicin (Invitrogen), 20 μg/ml.
They were routinely grown in a monolayer (30%-50% confluence) on a glass cover slide at 37°C in DMEM (Dulbecco's Modified Eagle Medium), supplemented with 1% L -glutamine, 1% HEPES [4-(2-hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)], 0.5% gentamicin, 1% penicillin/streptomycin and 10% Fetal Bovine Serum (Gibco, NY).
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