Words similar to genepix
Example sentences for: genepix
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Data were analyzed and displayed with Cluster and Treeview [ 31], AMAD [ 41], Genepix PRO (Axon Instruments), and Microsoft Excel.
All probes on these microarrays are 30-mer oligonucleotides spotted by piezoelectric technologies and covalently attached to a polymeric matrix [ 6 ] . These microarrays were hybridized, washed, and processed using a direct detection method of the biotin-containing transcripts by a Streptavidin-Alexa647 conjugate as previously described [ 6 ] . Processed slides were scanned using an Axon GenePix Scanner with the laser set to 635 nm, a PMT voltage of 600, and a scan resolution of 10 microns.
Microarrays with approximately 43,000 sequence-validated PCR-amplified human cDNAs representing 32,968 UniGene clusters were manufactured as described [ 32 ] . Hybridizations were performed using equal amounts of Cy3- and Cy5-labeled cDNAs according to previously published protocols [ 32 ] . Hybridized microarrays were scanned using a GenePix4000 array scanner and analyzed with GenePix Pro 3.0 software (Axon Instruments, Union City, CA).
Microarrays were scanned in both Cy3 and Cy5 channels with an Axon GenePix scanner (Foster City, CA) with a 10 μm resolution.
Hybridized arrays were scanned at 10-μm resolution on a GenePix 4000 scanner (Axon Instruments) at variable PMT voltage to obtain maximal signal intensities with < 1% probe saturation.