Words similar to gac

Example sentences for: gac

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The Q758C variant was frequently found adjacent to the wild-type T7 RNA polymerase promoter (GACT at positions -11 through -8) but was also found adjacent to a single mutant promoter (GAC G ), double mutant promoters (GA TA , G T C A ), a triple mutant promoter (G TTA ), and a promoter in which all the residues differed from wild-type ( TGTA ). Overall, the 'generalist' polymerase appeared to prefer mutant promoters that contained a T at position -9, and G or A at -8 positions.
TT primer: CAG AGG TGA GAC AGG AGT GGA G
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
Point mutations were introduced into Rlk-GFP by a PCR based strategy [ 53] with oligonucleotides encoding the required mutations (Mutated residues are in bold): Y420F (GGAC GAT GAA T TC ATC AGT TCT TCT G), Y91F (GTC AAG GCT CTT T T T GAC TTC CTG CC).
CT adapter: AAC TGC AGG AGG GAC AGT TGA AGG AGG CA CC TCC GTG C