Example sentences for: fura-

How can you use “fura-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Briefly, cells were harvested and re-suspended in HBS containing 20 μM fura-2/AM.

  • Ca 2+-free HBS contained 0.3 mM EGTA and the same salts as HBS without added CaCl 2 . Fura-2 acetoxymethyl ester (fura-2/AM), ethidium bromide, YO-PRO-1 and POPO-3 were obtained from Molecular Probes (Eugene, OR, USA).

  • MTX produced an immediate, concentration-dependent increase in [Ca 2+] i . At the highest concentration of MTX examined (3 nM), [Ca 2+] i rapidly increased within 1-2 minutes from a mean ± SE resting level of 63 ± 4 nM to an apparent [Ca 2+] i value close to fura-2 saturation.

  • After 7 days in culture, cells were loaded with 1.5 μM fura-2 AM (Molecular Probes, Eugene, OR) in HBSS and 1 mg/ml bovine serum albumin (BSA) for 30 minutes at 22°C and washed in HBSS for 30 minutes at 22°C.

  • Previous studies on human skin fibroblasts showed that MTX had no effect on [Ca 2+] i , vital dye uptake, or fura-2 efflux when the cells were challenged in the absence of extracellular Ca 2+[ 8].


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