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Example sentences for: fura-

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Addition of MTX to fura-2 -loaded BAECs, suspended in a cuvette in normal Ca 2+-containing buffer at 37°C, produced a time-dependent increase in [Ca 2+] i (Fig 1, upper panel ). Addition of either U73122 or U73343, the inactive analog, ~3 min before MTX produced a concentration-dependent inhibition of the change in [Ca 2+] i . U73343 exhibited a 3-fold greater potency compared to U73122 (Fig 1, insets ). Since U73343 has no significant effect on PLC over the concentration range employed [ 14 25 26 27 ] , these results suggest that inhibition of the MTX-induced response is independent of PLC.
[Ca 2+] i was measured using the fluorescent indicator, fura-2, as previously described [ 35 ] . Experiments were performed with cells in the twelfth to twentieth passage and 2-3 days post-confluency.
Fura-2 acetoxymethyl ester (fura-2/AM) and ethidium bromide (EB) were obtained from Molecular Probes (Eugene, OR, USA).
Ca 2+-free HBS contained 0.3 mM EGTA and the same salts as HBS without added CaCl 2 . Fura-2 acetoxymethyl ester (fura-2/AM), ethidium bromide, YO-PRO-1 and POPO-3 were obtained from Molecular Probes (Eugene, OR, USA).
After 7 days in culture, cells were loaded with 1.5 μM fura-2 AM (Molecular Probes, Eugene, OR) in HBSS and 1 mg/ml bovine serum albumin (BSA) for 30 minutes at 22°C and washed in HBSS for 30 minutes at 22°C.