Example sentences for: fura

How can you use “fura” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with HHBSS containing 1 mM probenecid and incubated with 5 μM Fura2-AM (Calbiochem) in HHBSS, 1 mM probenecid, and 1% DMSO for 45 min at 37°C.

  • Single-cell imaging was performed on the Olympus system described above, but using a 40×, 1.35 NA oil immersion objective and a Fura2 dichroic mirror and emission filter (Chroma).

  • Fura2 image pairs illuminated at 340 and 380 nm were taken at 1 Hz.

  • The [Ca 2+] i increase is expressed as the ratio of the background-corrected Fura2 fluorescence at 340 and 380 nm [ 42 ] .

  • Cells were then washed with HHBSS containing 1 mM probenecid and imaged after a 30 min incubation for de-esterification of the Fura2-AM.


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