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Example sentences for: fractions

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Subcellular fractionation of RNA into cytoskeleton-associated and cytosolic fractions was performed as previously described [ 15 34 ] . In brief, HL-60 cells were harvested by centrifugation and resuspension in buffer A (10 mM Tris pH 7.6, 0.25 M sucrose, 25 mM KCl, 5 mM MgCl 2 ) with 0.1% Igepal nonionic detergent (Sigma).
2A; fractions 15-16), approximately 4 minutes after the 120-kDa L. dispar APN elutes on the same column [ 19 ] . The candidate cadherin-like receptor fraction eluted in fractions 9-11 at around 180 kDa (Fig.
To the cytosolic fractions (supernatants), glycerol was added to a final concentration of 40%.
RNA was isolated from both fractions and hybridized to nylon filter DNA arrays that contained 5184 gene or EST sequences.
The CD34 +cells called fibrocytes, which are present in monocyte fractions of human blood and develop a fibroblast morphology when grown on fibronectin, have features identical to those of circulating vascular endothelial cell progenitors [ 28] and are probably not BMPCs.