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Example sentences for: fractions

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Elutriation fractions containing more than 50% and less than 75% monocytes were concentrated by centrifugation at 1200 rpm for 5 min, and the cell pellets were combined, reconstituted in DMEM plus 20% sterile heat-inactivated FCS (hereafter referred to as complete medium, unless otherwise stated), counted in a hemocytometer, washed in DMEM medium, repelleted, resuspended to 5 × 10 6/ml, and dispensed into either plastic tissue-culture slides or glass chamber slides.
Because the sampling fractions from different strata varied [ 22 ] , individual cases carried different weight [ 23 ] and all calculations, including means and proportions, were performed using appropriate case weights.
In this regard, in 0.05% Triton X-100 lysates, many transmembrane proteins remain in the light membrane fractions of a sucrose gradient [ 46 47 ] . Although the role of the CD98 light chain was not addressed in the earlier study [ 29 ] , we assume that it may have played a key role.
To the cytosolic fractions (supernatants), glycerol was added to a final concentration of 40%.
5 μg nuclear and cytoplasmic fractions) by SDS-PAGE.