Words similar to fractionated

Example sentences for: fractionated

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After centrifugation, the gradient was fractionated in 200 ul aliquots and analyzed at A 260 using a Bio-Rad SmartSpec 3000.
GAGs and polyanions in the CM were fractionated by anion-exchange column chromatography followed by PEDF-affinity column chromatography (Fig.
All procedures were published previously [ 6 20 ] . In brief, protein samples of 25 μg in 2 × sodium dodecylsulfate loading buffer were fractionated by 10% sodium dodecylsulfate-polyacrylamide gel electrophoresis at 100 V. The proteins were transferred onto 0.45-μm pore nitrocellulose membrane (Schleicher & Schuell, Keene, NH) at 40 V for 45 to 60 minutes.
Bacterial cells were fractionated as previously described [ 18 ] . Briefly, bacterial cells were chilled on ice after growth, harvested by centrifugation, and washed in cold phosphate-buffered saline (PBS; [ 34 ] ). Bacterial whole cell fractions were prepared by resuspending the washed cells in cold PBS and precipitating total proteins with 10% (vol/vol) trichloroacetic acid (TCA) on ice overnight.
Total RNA (20 μg) was fractionated on 1% agarose gels containing formaldehyde and transferred to DURLON-UV (Stratagene) nylon membranes.