Example sentences for: fractionated

How can you use “fractionated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • NIH3T3 cells suspended in PBS were treated with 1 μM staurosporine, 1 μM calyculin A or 100 μM pervanadate and fractionated with 0.5% Triton X-100 or DOTMAC.

  • Bacterial cells were fractionated as previously described [ 18 ] . Briefly, bacterial cells were chilled on ice after growth, harvested by centrifugation, and washed in cold phosphate-buffered saline (PBS; [ 34 ] ). Bacterial whole cell fractions were prepared by resuspending the washed cells in cold PBS and precipitating total proteins with 10% (vol/vol) trichloroacetic acid (TCA) on ice overnight.

  • Total RNA (20 μg) was fractionated on 1% agarose gels containing formaldehyde and transferred to DURLON-UV (Stratagene) nylon membranes.

  • Digested DNA was size fractionated via gel electrophoresis in 12 cm gels containing 0.8% agarose run in IX TAE or TBE at 25 volts for 12 hours.

  • To confirm these localization patterns, cells derived from the epitope-tagged strains were biochemically fractionated into cytoplasmic and nuclear components.


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