Example sentences for: fractionated

How can you use “fractionated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • NIH3T3 cells suspended in PBS were treated with 1 μM staurosporine, 1 μM calyculin A or 100 μM pervanadate and fractionated with 0.5% Triton X-100 or DOTMAC.

  • Subcellular fractions were fractionated over a 25 ml Superose-6 FPLC column (Pharmacia) with 1× gel filtration buffer (25 mM Tris/HCl, pH 7.5, 220 mM NaCl, 1 mM MgCl 2 , 1 μM ZnSO 4 ) at a flow rate of 0.2 ml/min.

  • RNA was denatured with glyoxal, fractionated via agarose gel electrophoresis, and immobilized on a positively charged nylon membrane.

  • Fractionated DNAs were transferred to Hybond-XL membrane (Amersham Pharmacia Biotech, Piscataway, NJ) as described by Sambrook et al.

  • After 10 min on ice, lysates were centrifuged (4000 × g, 5 min) and supernatants containing 10 μg of protein fractionated on 10% SDS polyacrylamide gels.


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