Words similar to fractionated

Example sentences for: fractionated

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After first- and second-strand synthesis, the full-length cDNAs were selected, size fractionated and cloned into pBluescript.
Bacterial cells were fractionated as previously described [ 18 ] . Briefly, bacterial cells were chilled on ice after growth, harvested by centrifugation, and washed in cold phosphate-buffered saline (PBS; [ 34 ] ). Bacterial whole cell fractions were prepared by resuspending the washed cells in cold PBS and precipitating total proteins with 10% (vol/vol) trichloroacetic acid (TCA) on ice overnight.
PCR products were then fractionated on 2.5% agarose gels.
GAGs and polyanions in the CM were fractionated by anion-exchange column chromatography followed by PEDF-affinity column chromatography (Fig.
6 mM) (Sigma) dissolved in formamide was added to the reaction following PCR, and 0.6 μl of the product was fractionated on denaturing acrylamide gels (6.