Words similar to fractionated

Example sentences for: fractionated

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Fractionated DNA in gels was depurinated, denatured, and neutralized according to standard protocols [ 76 ] . DNA was transferred to positively charged nylon membranes (Hybond N +) (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) by the wick transfer method using 20X SSC overnight.
To confirm these localization patterns, cells derived from the epitope-tagged strains were biochemically fractionated into cytoplasmic and nuclear components.
Digested DNA was size fractionated via gel electrophoresis in 12 cm gels containing 0.8% agarose run in IX TAE or TBE at 25 volts for 12 hours.
All procedures were published previously [ 6 20 ] . In brief, protein samples of 25 μg in 2 × sodium dodecylsulfate loading buffer were fractionated by 10% sodium dodecylsulfate-polyacrylamide gel electrophoresis at 100 V. The proteins were transferred onto 0.45-μm pore nitrocellulose membrane (Schleicher & Schuell, Keene, NH) at 40 V for 45 to 60 minutes.
To examine whether mactinin was present in immune complexes in rheumatoid arthritis fluid, some samples were acidified by dialysis against 0.1 M sodium acetate, pH 4.1, to dissociate complexes [ 18 ] . The acidified samples were first fractionated on an 800 ml G-75 Sephadex (Pharmacia, Piscataway, NJ) size-exclusion column.