Words similar to fractionation

Example sentences for: fractionation

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The serine/threonine protein phosphatase type 5 (PP5) was purified from bovine brain according to the following procedure: a bovine brain was homogenized in ~ 3 volumes of buffer A (50 mM HEPES pH 7.5 4°C, 1 mM EDTA, 10 mM PMSF and 0.1% 2-mercaptoethanol) and subjected to centrifugation at 15,000 g for 40 min. followed by a 45%-60% ammonium sulfate fractionation of the supernatant.
Our previous fractionation of mouse testis extracts showed that most DAZL were present in the post mitochondrial fraction, and some of them were associated with polyribosomes [ 12 ] . Similar analyses showed that a majority of DAZAP1 in adult mouse testes was also present in the cytoplasmic fraction (data not shown).
Detection of nmt55/p54 nrbby Western blot analyses requires tumor pulverization, homogenization, fractionation and the preparation of cellular extracts.
Tissue preparation and tubulin fractionation
Upon such fractionation, we observe the Src family kinase Fyn constitutively associated with the RAFT fraction (Fig.