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Example sentences for: fosmids
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Orthologous regions of the D. melanogaster genome corresponding to sequenced fosmids were identified using default parameters of BLAT [ 78] against a database made up of the Release 3 sequences [ 9].
The lengths of fosmids sequenced for the eight candidate regions are shown in Table 1. Though we were able to obtain species-specific STSs for the D. willistoni twi gene, we were not able to obtain clones for this region from the D. willistoni fosmid library.
In the 30 fosmids, we predict a total of 164 protein-coding genes in non-melanogaster species (53 in D. erecta , 41 in D. pseudoobscura , 39 in D. willistoni , 31 in D. littoralis ) that form orthologous clusters with 81 D. melanogaster genes.
In general, our data indicate that the microsyntenic order of coding and non-coding sequences is highly conserved in the genus Drosophila at the scale of individual fosmids (approximately 40 kb).