Words similar to fosmid

Example sentences for: fosmid

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On the basis of genome size considerations and the desire to investigate a range of divergence times in the genus Drosophila , we constructed fosmid libraries (approximately 40-kb inserts) for D. erecta, D. pseudoobscura, D. willistoni and D. littoralis (Figure 1).
To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).
We designed degenerate PCR primers for a set of eight well-characterized genes ( apterous (ap), even-skipped (eve), fushi-tarazu (ftz), twist (twi) , and Rhodopsins 1, 2, 3 and 4 ( Rh1, Rh2, Rh3 and Rh4 )) to obtain species-specific sequence-tagged sites (STSs) that were subsequently used for hybridization to gridded fosmid filters (see Materials and methods).
Detailed information about the Drosophila stocks, construction of the genomic fosmid libraries and their availability can be found at the Children's Hospital of Oakland Research Institute BACPAC Resources website [ 75].
In total, 30 fosmid clones were isolated and sequenced using methods described in [ 9] which sum to 1,257,069 bp.