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Example sentences for: fosmid

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To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).
Coding sequences in these fosmid clones were predicted using the computational pipeline used to re-annotate the D. melanogaster genome [ 12].
Microsynteny is generally maintained at the scale of individual fosmid clones, and the few rearrangement breakpoints, transposable elements and gene transpositions can readily be identified.
Detailed information about the Drosophila stocks, construction of the genomic fosmid libraries and their availability can be found at the Children's Hospital of Oakland Research Institute BACPAC Resources website [ 75].
On the basis of genome size considerations and the desire to investigate a range of divergence times in the genus Drosophila , we constructed fosmid libraries (approximately 40-kb inserts) for D. erecta, D. pseudoobscura, D. willistoni and D. littoralis (Figure 1).