Words similar to fosmid

Example sentences for: fosmid

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Coding sequences in these fosmid clones were predicted using the computational pipeline used to re-annotate the D. melanogaster genome [ 12].
Construction of Drosophilaspecies fosmid libraries
The 30 non-melanogaster fosmid (and the D. virilis ap P1) sequences were computationally processed using the pipeline used to re-annotate the D. melanogaster genome [ 12].
On the basis of genome size considerations and the desire to investigate a range of divergence times in the genus Drosophila , we constructed fosmid libraries (approximately 40-kb inserts) for D. erecta, D. pseudoobscura, D. willistoni and D. littoralis (Figure 1).
To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).