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Example sentences for: fosmid

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The lengths of fosmids sequenced for the eight candidate regions are shown in Table 1. Though we were able to obtain species-specific STSs for the D. willistoni twi gene, we were not able to obtain clones for this region from the D. willistoni fosmid library.
To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).
Microsynteny is generally maintained at the scale of individual fosmid clones, and the few rearrangement breakpoints, transposable elements and gene transpositions can readily be identified.
We designed degenerate PCR primers for a set of eight well-characterized genes ( apterous (ap), even-skipped (eve), fushi-tarazu (ftz), twist (twi) , and Rhodopsins 1, 2, 3 and 4 ( Rh1, Rh2, Rh3 and Rh4 )) to obtain species-specific sequence-tagged sites (STSs) that were subsequently used for hybridization to gridded fosmid filters (see Materials and methods).
We can directly confirm the nature of one rearrangement ( D. littoralis ftz ) as a paracentric micro-inversion since both breakpoints are contained within a single fosmid clone.