Example sentences for: fosmid

How can you use “fosmid” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We designed degenerate PCR primers for a set of eight well-characterized genes ( apterous (ap), even-skipped (eve), fushi-tarazu (ftz), twist (twi) , and Rhodopsins 1, 2, 3 and 4 ( Rh1, Rh2, Rh3 and Rh4 )) to obtain species-specific sequence-tagged sites (STSs) that were subsequently used for hybridization to gridded fosmid filters (see Materials and methods).

  • To construct fosmid libraries, genomic DNA from adult flies was prepared by partial digestion with Mbo I and size-selecting fragments using pulsed-field gel electrophoresis, then cloned in the Bam HI site of the fosmid vector pFOS1 [ 74] and transformed into Escherichia coli strain XL1-Blue MR (Stratagene).

  • The 30 non-melanogaster fosmid (and the D. virilis ap P1) sequences were computationally processed using the pipeline used to re-annotate the D. melanogaster genome [ 12].

  • Detailed information about the Drosophila stocks, construction of the genomic fosmid libraries and their availability can be found at the Children's Hospital of Oakland Research Institute BACPAC Resources website [ 75].

  • Microsynteny is generally maintained at the scale of individual fosmid clones, and the few rearrangement breakpoints, transposable elements and gene transpositions can readily be identified.


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