Example sentences for: flag-mhck-c

How can you use “flag-mhck-c” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • If FLAG-MHCK-C was not subjected to a pre-autophosphorylation step, 32P incorporation into the peptide displayed a similar lag phase as observed for myosin II phosphorylation (Figure 4Aand 4B, open symbols).

  • FLAG-MHCK-C purification and phosphorylation assays

  • max of the enzyme 50-fold [ 25 ] . To test for similar autophosphorylation regulation of MHCK-C, we tested the activity of FLAG-MHCK-C with and without an initial autophosphorylation step, towards the peptide substrate MH-1 (a 16-residue peptide corresponding to one of the mapped MHC phosphorylation target sites for MHCK A in the myosin tail).

  • Another feature reported earlier for MHCK-A activation is that myosin II itself stimulates autophosphorylation [ 25 ] . To test whether MHCK-C autophosphorylation is accelerated in the presence of myosin II, the stoichiometry of FLAG-MHCK-C autophosphorylation was evaluated in the presence and absence of myosin II filaments.

  • Purified FLAG-MHCK-C was subjected to SDS-PAGE.


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