Words similar to flag-mhck-c

Example sentences for: flag-mhck-c

How can you use “flag-mhck-c” in a sentence? Here are some example sentences to help you improve your vocabulary:

The ammonium sulfate precipitate, containing the FLAG-MHCK-C protein, was collected by centrifugation and resuspended with gentle douncing in 20 ml TBS containing 1 mM EDTA (TBSE), and 1X PIC1 and PIC2.
As reported previously for expression of MHCK-A [ 24 ] , this selection process resulted in cell lines with increased expression level of FLAG-MHCK-C, several-fold higher than the initial expression level.
For purification of FLAG-MHCK-C, 8-10 liters of 3xALA/pTX-MKC2 cells were propagated in suspension culture in HL-5 medium to approximately 5 × 10 6cells/ml.
To facilitate FLAG-MHCK-C protein purification, Ax2/pTX-MKC2 cell lines were then subjected to incremental increases in G418 selection level over approximately 3 weeks, to a final selection level of 40 μg/ml.
Dictyostelium myosin II was purified as described [ 22 ] . Phosphorylation reactions with myosin II and FLAG-MHCK-C were performed in 20 mM TES pH 7, 2 mM MgCl 2 , and 0.5 mM ATP, with 32P-γ-ATP included at 800 Ci/mol final concentration.