Words similar to flag-mhck-c

Example sentences for: flag-mhck-c

How can you use “flag-mhck-c” in a sentence? Here are some example sentences to help you improve your vocabulary:

In previous work, when this method was applied to MHCK-A-expressing cell lines the elevated expression of MHCK-A resulted in myosin II hyperphosphorylation and myosin II filament disassembly, and corresponding loss of ability of cells to grow in suspension [ 24 ] . We observed the same effect in the current studies in attempting to force high expression of FLAG-MHCK-C.
FLAG-MHCK-C purification and phosphorylation assays
To facilitate FLAG-MHCK-C protein purification, Ax2/pTX-MKC2 cell lines were then subjected to incremental increases in G418 selection level over approximately 3 weeks, to a final selection level of 40 μg/ml.
max of the enzyme 50-fold [ 25 ] . To test for similar autophosphorylation regulation of MHCK-C, we tested the activity of FLAG-MHCK-C with and without an initial autophosphorylation step, towards the peptide substrate MH-1 (a 16-residue peptide corresponding to one of the mapped MHC phosphorylation target sites for MHCK A in the myosin tail).
The FLAG-MHCK-C protein consistently suffered proteolytic cleavage during purification.