Words similar to flag-mhck-c

Example sentences for: flag-mhck-c

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Purified FLAG-MHCK-C readily phosphorylated native D. discoideum myosin II on the myosin heavy chain (MHC) in vitro (Figure 2Band 2C).
For purification of FLAG-MHCK-C, 8-10 liters of 3xALA/pTX-MKC2 cells were propagated in suspension culture in HL-5 medium to approximately 5 × 10 6cells/ml.
Purified FLAG-MHCK-C was subjected to SDS-PAGE.
If FLAG-MHCK-C was pretreated with Mg-ATP for 10 min at room temperature, the lag phase for peptide phosphorylation was eliminated (figure 4Aand 4B, closed symbols).
Dictyostelium myosin II was purified as described [ 22 ] . Phosphorylation reactions with myosin II and FLAG-MHCK-C were performed in 20 mM TES pH 7, 2 mM MgCl 2 , and 0.5 mM ATP, with 32P-γ-ATP included at 800 Ci/mol final concentration.