Words similar to flag-mhck-c

Example sentences for: flag-mhck-c

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If FLAG-MHCK-C was not subjected to a pre-autophosphorylation step, 32P incorporation into the peptide displayed a similar lag phase as observed for myosin II phosphorylation (Figure 4Aand 4B, open symbols).
The FLAG-MHCK-C protein consistently suffered proteolytic cleavage during purification.
Another feature reported earlier for MHCK-A activation is that myosin II itself stimulates autophosphorylation [ 25 ] . To test whether MHCK-C autophosphorylation is accelerated in the presence of myosin II, the stoichiometry of FLAG-MHCK-C autophosphorylation was evaluated in the presence and absence of myosin II filaments.
The ammonium sulfate precipitate, containing the FLAG-MHCK-C protein, was collected by centrifugation and resuspended with gentle douncing in 20 ml TBS containing 1 mM EDTA (TBSE), and 1X PIC1 and PIC2.
For purification of FLAG-MHCK-C, 8-10 liters of 3xALA/pTX-MKC2 cells were propagated in suspension culture in HL-5 medium to approximately 5 × 10 6cells/ml.