Words similar to flag-mhck-c

Example sentences for: flag-mhck-c

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max of the enzyme 50-fold [ 25 ] . To test for similar autophosphorylation regulation of MHCK-C, we tested the activity of FLAG-MHCK-C with and without an initial autophosphorylation step, towards the peptide substrate MH-1 (a 16-residue peptide corresponding to one of the mapped MHC phosphorylation target sites for MHCK A in the myosin tail).
First, FLAG-MHCK-C autophosphorylates during the course of in vitro phosphorylation reactions (Figure 2B).
Another feature reported earlier for MHCK-A activation is that myosin II itself stimulates autophosphorylation [ 25 ] . To test whether MHCK-C autophosphorylation is accelerated in the presence of myosin II, the stoichiometry of FLAG-MHCK-C autophosphorylation was evaluated in the presence and absence of myosin II filaments.
Second, the activity of FLAG-MHCK-C appears to be very low in the initial stages of in vitro phosphorylation reactions, but then rises after approximately 5 minutes (Figure 2C).
Purified FLAG-MHCK-C was subjected to SDS-PAGE.