Words similar to flag-mhck-c

Example sentences for: flag-mhck-c

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The ammonium sulfate precipitate, containing the FLAG-MHCK-C protein, was collected by centrifugation and resuspended with gentle douncing in 20 ml TBS containing 1 mM EDTA (TBSE), and 1X PIC1 and PIC2.
max of the enzyme 50-fold [ 25 ] . To test for similar autophosphorylation regulation of MHCK-C, we tested the activity of FLAG-MHCK-C with and without an initial autophosphorylation step, towards the peptide substrate MH-1 (a 16-residue peptide corresponding to one of the mapped MHC phosphorylation target sites for MHCK A in the myosin tail).
If FLAG-MHCK-C was pretreated with Mg-ATP for 10 min at room temperature, the lag phase for peptide phosphorylation was eliminated (figure 4Aand 4B, closed symbols).
Second, the activity of FLAG-MHCK-C appears to be very low in the initial stages of in vitro phosphorylation reactions, but then rises after approximately 5 minutes (Figure 2C).
As reported previously for expression of MHCK-A [ 24 ] , this selection process resulted in cell lines with increased expression level of FLAG-MHCK-C, several-fold higher than the initial expression level.