Words similar to flag-cop

Example sentences for: flag-cop

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To further investigate whether COP1 itself undergoes ubiquitination, a His-tagged ubiquitin expression construct (His-Ub) was co-transfected into the 293 cells with FLAG-COP1, followed by His purification under denaturing condition (in 8 M Urea).
Immunoblot analysis of the immunoprecipitates with anti-HA antibodies detected multiple distinct bands and smear only when FLAG-COP1 and HA-Ub were co-expressed, indicating that at least a subset of COP1 associated proteins are ubiquitinated in human cells (Fig.
Though FLAG-N280 was not as heavily ubiquitinated as the full-length FLAG-COP1, it clearly showed a ladder pattern, in which the size difference between neighboring bands of the ladder was consistent with the unit molecular weight of His-Ub (Fig.
Similarly, FLAG-COP1 is also remained on the NE after Triton extraction (data not shown).
Immunoblot analysis of the precipitates with anti-FLAG antibodies detected a higher molecular weight smear only upon co-expression of FLAG-COP1 and His-Ub, therefore confirming that COP1 is indeed a ubiquitination substrate (Fig.