Example sentences for: flag-cop

How can you use “flag-cop” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To further investigate whether COP1 itself undergoes ubiquitination, a His-tagged ubiquitin expression construct (His-Ub) was co-transfected into the 293 cells with FLAG-COP1, followed by His purification under denaturing condition (in 8 M Urea).

  • Immunoblot analysis of the immunoprecipitates with anti-HA antibodies detected multiple distinct bands and smear only when FLAG-COP1 and HA-Ub were co-expressed, indicating that at least a subset of COP1 associated proteins are ubiquitinated in human cells (Fig.

  • Though FLAG-N280 was not as heavily ubiquitinated as the full-length FLAG-COP1, it clearly showed a ladder pattern, in which the size difference between neighboring bands of the ladder was consistent with the unit molecular weight of His-Ub (Fig.

  • Similarly, FLAG-COP1 is also remained on the NE after Triton extraction (data not shown).

  • Similar results were obtained when other cell lines (including HeLa, NIH3T3, and CHO cell lines) were used or immuno-fluorescence studies were carried out with FLAG-COP1 instead of GFP-COP1 (data not shown).


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