Words similar to flag-tagged

Example sentences for: flag-tagged

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For example, the anti-Flag spots showed good quantitation of Flag-tagged Bacterial Alkaline Phosphatase (Flag-BAP) down to an absolute concentration of 1 ng/ml and a partial concentration of 10 -6, when measured in solution at a total protein concentration of 600 μg/ml.
To test whether artificially increasing the level of TAF II 135 would affect the proper differentiation of F9 cells, we established cell lines which constitutively express flag-tagged versions of human TAF II 135 [fTAF II 135(372-1083) and fTAF II 135(805-1083) see Materials and methods].
Similar observations were made when other cell lines were used (HeLa, NIH3T3 and CHO cells) or by using FLAG-tagged construct series instead of GFP fusions (data not shown).
After immunoprecipitation of the FLAG-tagged δ-OR or the EGFR with the appropriate antibodies, we found that only DSLET induced tyrosine phosphorylation of the δ-OR, whereas only EGF potentiated EGFR phosphorylation (Figure 4).
Since mature β subunits are only found in the 20S proteasome [ 19 ] , the detection of the mature form of the Flag-tagged HsN3 assured us that the exogenous Flag-tagged HsN3 proteins are normal in their ability to be incorporated into the 20S proteasome.