Words similar to flag-tagged
Example sentences for: flag-tagged
How can you use “flag-tagged” in a sentence? Here are some example sentences to help you improve your vocabulary:
A FLAG-tagged DEK adenoviral vector constructed by the University of Michigan Vector Core was used to transduce T98G cells (ATCC) by incubation for 48 hours before harvesting for immunoprecipitation.
HEK-293 cells were transfected, in house, with the cDNA encoding the FLAG-tagged wild-type (HEK-δOR) mouse δ-OR.
For example, the anti-Flag spots showed good quantitation of Flag-tagged Bacterial Alkaline Phosphatase (Flag-BAP) down to an absolute concentration of 1 ng/ml and a partial concentration of 10 -6, when measured in solution at a total protein concentration of 600 μg/ml.
Similar observations were made when other cell lines were used (HeLa, NIH3T3 and CHO cells) or by using FLAG-tagged construct series instead of GFP fusions (data not shown).
After immunoprecipitation of the FLAG-tagged δ-OR or the EGFR with the appropriate antibodies, we found that only DSLET induced tyrosine phosphorylation of the δ-OR, whereas only EGF potentiated EGFR phosphorylation (Figure 4).