Words similar to flag-tagged
Example sentences for: flag-tagged
How can you use “flag-tagged” in a sentence? Here are some example sentences to help you improve your vocabulary:
To test whether artificially increasing the level of TAF II 135 would affect the proper differentiation of F9 cells, we established cell lines which constitutively express flag-tagged versions of human TAF II 135 [fTAF II 135(372-1083) and fTAF II 135(805-1083) see Materials and methods].
HEK-293 cells were transfected, in house, with the cDNA encoding the FLAG-tagged wild-type (HEK-δOR) mouse δ-OR.
As another line of experiments, we generated expression constructs containing N-terminal GFP- and FLAG-tagged MmCOP1 and HsCOP1.
Similar observations were made when other cell lines were used (HeLa, NIH3T3 and CHO cells) or by using FLAG-tagged construct series instead of GFP fusions (data not shown).
After immunoprecipitation of the FLAG-tagged δ-OR or the EGFR with the appropriate antibodies, we found that only DSLET induced tyrosine phosphorylation of the δ-OR, whereas only EGF potentiated EGFR phosphorylation (Figure 4).
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