Example sentences for: facscalibur

How can you use “facscalibur” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing the cells twice in Dulbecco's phosphate buffered saline supplemented with 2% FCS and fixation in 1% paraformaldehyde (Ph = 7.4), samples were analyzed by flow cytometry using FACScalibur (Becton Dickinson, San Jose, CA) instrument.

  • After 30-min incubation at 4°C, cells were washed twice, resuspended in staining buffer and kept on ice before analysis on a flow cytometer (FACSCalibur™, Becton Dickinson Immunocytometry Systems).

  • After washing with 1 ml of staining buffer, the cells were fixed with 600 ul of 0.5% PFA for approximately 1 day and then analyzed with a FACSCalibur flow cytometer (Becton-Dickinson) equipped with a FL4 channel.

  • Cells were incubated at room temperature for 30 min, washed, then analyzed using a two-laser, four-color FACSCalibur (Becton Dickinson, Franklin Lakes, New Jersey, United States) or sorted using a FACSVantage flow cytometer (Becton Dickinson).

  • Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).


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