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Example sentences for: facscalibur
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Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).
After washing with 1 ml of staining buffer, the cells were fixed with 600 ul of 0.5% PFA for approximately 1 day and then analyzed with a FACSCalibur flow cytometer (Becton-Dickinson) equipped with a FL4 channel.
Flow cytometry was also used to assess GC viability and size, using methods described previously in detail to determine propidium iodide uptake and forward (small angle light) scatter, respectively [ 18 25 ] . Briefly, ten thousand cells were examined per sample using a Becton Dickinson FACSCalibur to excite the cells with a 488 nM argon laser, and the collected data obtained were analyzed using Cell Quest™ software (Becton Dickinson, Mount View, CA).
Parallel samples were processed using standard four-color flow cytometry on a Becton Dickinson FACSCalibur, using the MultiTEST reagents and TruCOUNT beads, and analyzed using MultiSET software.
Flow cytometry was performed using a FACSCalibur (Becton Dickinson) and analyzed using CellQuest software (Becton Dickinson).
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