Example sentences for: facscalibur

How can you use “facscalibur” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing the cells twice in Dulbecco's phosphate buffered saline supplemented with 2% FCS and fixation in 1% paraformaldehyde (Ph = 7.4), samples were analyzed by flow cytometry using FACScalibur (Becton Dickinson, San Jose, CA) instrument.

  • Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).

  • Flow cytometry was also used to assess GC viability and size, using methods described previously in detail to determine propidium iodide uptake and forward (small angle light) scatter, respectively [ 18 25 ] . Briefly, ten thousand cells were examined per sample using a Becton Dickinson FACSCalibur to excite the cells with a 488 nM argon laser, and the collected data obtained were analyzed using Cell Quest™ software (Becton Dickinson, Mount View, CA).

  • Cells were incubated at room temperature for 30 min, washed, then analyzed using a two-laser, four-color FACSCalibur (Becton Dickinson, Franklin Lakes, New Jersey, United States) or sorted using a FACSVantage flow cytometer (Becton Dickinson).

  • We compared results from our microchip assay with results available from flow cytometry, the latter obtained on a FACSCalibur through standard clinical laboratory operating procedures.


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