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Example sentences for: facs
How can you use “facs” in a sentence? Here are some example sentences to help you improve your vocabulary:
5B; for examples of actual FACS analyses, please, see additional files: Additional data #3.ppt) - a considerable number of which were α-SMA positive.
Cells with advanced apoptosis have high fluorescence and were found to the right of the broken vertical bar in b, b' . During serum starvation about 10% of PP fibroblasts were found floating (data not shown, see also [ 36 ] ). FACS analysis demonstrated that these cells were all apoptotic with FL1 between 100-200 - i.e. around and to the right of the dash in ( b, b' ). Corresponding graphs are given in Fig.
Cells were isolated directly from PBMCs from patient 422 by FACS as described above.
Treatment of L1210/CPA cells in vitro with 50 μM DEAB abolished the tumor cells' resistance to 4-HC [ 19 ] . DEAB also exhibited in vivo efficacy as a ALDH1A1 inhibitor, as demonstrated by the toxicity to the intestinal crypt cells in mice receiving co-injections of DEAB and cyclophosphamide [ 21 ] . More recently, DEAB has been used to demonstrate that overexpression of human ALDH1A1 in transfected cell lines is sufficient to cause cellular resistance to the oxazaphosphorines [ 22 ] and 4-HC [ 23 ] . DEAB has also been an effective reagent in fluorescent-activated cell sorting (FACS) techniques to isolate human hematopoietic stem cells based on the expression of ALDH1A1 activity [ 5 6 ] . In contrast to DEAB and DPAB, daidzin - a natural product isolated from Pueraria lobata (the Kudzu plant) - shows specificity for inhibiting ALDH2 at concentrations similar to that of DPAB used to inhibit ALDH1A1 [ 24 25 ] . An inhibitor specific for ALDH1A2 has not yet been reported.
FACS analysis demonstrated that these cells were all apoptotic (not shown, see also [ 36 ] ). Although TGFβ1 acted anti-apoptotically in PP cultures (palmar and normal plantar fibroblasts) and prevented the shedding of cells into the media, its effect was dissimilar in non-PP cultures, where TGFβ1 had either no impact (keloid T and normal nonplantar fibroblasts) or was pro-apoptotic (keloid D cultures).
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