Example sentences for: exgen

How can you use “exgen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • HeLa cells were grown in DMEM (Gibco/BRL) supplemented with 10% fetal calf serum, seeded at 10 cells/well in 96-well microtiter plates and transfected 8 hours later using Exgen 500 (Euromedex, Souffelweyersheim, France).

  • Various concentrations of plasmid and PEI (ExGen 500, MBI Fermentas, Hanover, MD) were prepared, using 150 mM NaCl in sterile water as the diluent.

  • As the number of protonatable nitrogens on the linear 22 kD ExGen 500 PEI polymer at physiological pH is roughly equal to 5.47 nmol per μg PEI, and 1 μg DNA corresponds to 3 nmol phosphate groups, this means that the peak yield observed was at a PEI nitrogen: DNA phosphate (N/P) ratio of ∼ 9. Thus, the most suitable N/P ratio for in vitro sympathetic neurons appears to be around 9, creating positively charged DNA/PEI complexes [ 17].


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