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Example sentences for: epr
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Characterization of EPR species from VO 2+-AMPCP bound to sCD39
The parallel transitions of bound VO 2+-EPR spectrum are shown in Figure 6. The features derived from the VO 2+-EPR spectrum were 486 MHz for A || and 1.946 for g || , which was designed as species V (Fig.
In the presence of AMPCP, bound VO 2+produced two EPR features, species D1 and species D2 that are separated by about 30 MHz.
However, intact CD39 does not release intermediate ADP during ATP hydrolysis, suggesting that there is only one ADP binding site on each CD39 monomer in the intact protein [ 2 25 ] . The two EPR species observed with VO 2+-AMPCP probably correspond to the two different conformations of bound ADP at the same catalytic site on sCD39.
CW-EPR experiments were carried out at X-band (9 GHz) using a Bruker 300E spectrometer with a TE102 rectangular standard cavity and a liquid nitrogen flow cryostat operating at 150 K. Simulations of these EPR spectra were accomplished with the computer program QPOWA [ 30 31 ] ).