Words similar to epitope-tagged

Example sentences for: epitope-tagged

How can you use “epitope-tagged” in a sentence? Here are some example sentences to help you improve your vocabulary:

Size exclusion chromatography of lysates prepared from cells harboring epitope-tagged Pcu3p or CSN subunits at the endogenous genomic loci, revealed precise co-elution of Pcu3p with CSN subunits 1, 3, 4, and 5 in high molecular weight fractions corresponding to approximately 550 kDa (Fig.
As a reference for SCF Popcomplex formation, we used strains carrying 13Myc epitope-tagged Pop1p or Pop2p integrated into the genome of wild-type cells.
These effects on epitope-tagged Smo protein were observed in multiple lines (data not shown).
Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .
Since the substrates of putative SCF Pop1pand SCF Pop2pubiquitin ligases are unknown, we adopted a substrate-independent assay originally described by Lyapina et al . [ 27 ] . For this experiment, we again used the strains harboring genomically integrated Myc epitope-tagged Pop1p or Pop2p in a background deficient in the respective heterooligomerization partner ( Δpop2 pop1-13myc , Δpop1 pop2-13myc strains).