Example sentences for: epitope-tagged

How can you use “epitope-tagged” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .

  • For this, we created a pop2 deletion strain carrying Pop1p modified with 13 Myc epitope tags at the endogenous genomic locus ( pop1-13myc Δpop2 strain ). In addition, we created the reciprocal pop2-13myc Δpop1 strain containing epitope-tagged endogenous Pop2p in a pop1 deletion background.

  • In order to evaluate the transcriptional activity of FLAG epitope-tagged β-catenin in a medulloblastoma cell line, the S37F and wild-type constructs were introduced into DAOY cells along with the Wnt pathway TOPFLASH reporter.

  • To confirm these localization patterns, cells derived from the epitope-tagged strains were biochemically fractionated into cytoplasmic and nuclear components.

  • Deletion strains and epitope-tagged strains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 32].


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