Words similar to epitope-tagged
Example sentences for: epitope-tagged
How can you use “epitope-tagged” in a sentence? Here are some example sentences to help you improve your vocabulary:
In the transgenic line expressing high levels of human wild-type β-catenin, significant amounts of FLAG epitope-tagged protein were detected, however overall β-catenin levels were only slightly increased, suggesting the mechanisms that normally regulate β-catenin level were able to compensate for the addition of the human transgenic protein.
For this, we created a pop2 deletion strain carrying Pop1p modified with 13 Myc epitope tags at the endogenous genomic locus ( pop1-13myc Δpop2 strain ). In addition, we created the reciprocal pop2-13myc Δpop1 strain containing epitope-tagged endogenous Pop2p in a pop1 deletion background.
Similar results were reported for this FLAG epitope-tagged mutant in other cell lines [ 26 ] . Second, the tail kinking and "waltzing" phenotypes in our transgenic animals expressing stabilized human β-catenin are highly similar to those seen in Fused mice [ 29 ] . Fused encodes the murine Axin homologue, and mutation of this gene results in activation of Wnt signaling [ 30 ] . The tail kinking in our animals presumably results from low level expression of the mutant transgene in utero during vertebral patterning.
To determine whether phosphorylated Rum1p interacts with Pop1p and Pop2p, protein lysate was prepared from cells co-overexpressing epitope-tagged combinations of Pop1p and Pop2p.
Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .
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