Words similar to eluted

Example sentences for: eluted

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The PCR products were purified with Qiagen PCR Purification Kit (Qiagen, Valencia, CA) and eluted into 42 μl of ddH 2 O, which was used for indirect labeling with PCR.
Binding studies of recombinant RGS3-GST fusion protein to 35S-Met-Gα-proteins were performed as detailed [ 9 ] . Glutathione-coupled beads were used to select 35S-Gα proteins bound to RGS3-GST, and were then eluted for separation by SDS/PAGE [ 9 ] . The amount and molecular size of 35S-Gα protein were determined using a Phosphor imaging device (Molecular Dynamics).
FLAG-DEK was immunoprecipitated using anti-FLAG resin (Sigma-Aldrich, St Louis, MO, USA) in accordance with the manufacturer's instructions and was eluted by competition with peptide containing three FLAG recognition epitopes.
First-strand cDNA was purified from unincorporated amino allyl-dUTPs on QIAquick PCR purification columns (Qiagen, Valencia, CA) according to manufacturer's instructions, except that QIAquick wash buffer was replaced with 5 mM K +phosphate buffer (pH 8.5) containing 80% ethanol, and cDNA was eluted with 4 mM K +phosphate buffer (pH 8.5).
Two peaks with aromatic aminotransferase activity eluted from the hydroxylapatite column mentioned above.