Example sentences for: eluted

How can you use “eluted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • It was then loaded on a 10 cm × 1 cm hydroxylapatite (BioGel HTP, BioRad) column and eluted under pressure with a 0-600 mM phosphate gradient (200 mL total volume, flow rate 0.5 ml/min).

  • The purified proteins were eluted with reduced glutathione at 1 mg/ml in elution buffer (100 mM Tris (pH 7.9), 120 mM NaCl).

  • The biotinylated Tat proteins were purified on a column of soft release avidin resin, cleaved from the fusion protein using factor Xa and eluted from the column followed by desalting with a PD10 column.

  • 2. GSTCdc6 eluted from the beads following high salt wash failed to give any band shift suggesting that the ~35 kDa polypeptide was responsible for the DNA binding activity.

  • The eluted fractions were spun through 0.22 μM Millex filters (Millipore), dialyzed for 6 hours at 4°C in dialysis buffer (20 mM Tris (pH 7.9), 100 mM KCl, 20% (v/v) glycerol, and 1 mM DTT), frozen in liquid nitrogen, and stored at -80°C.


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