Words similar to eluted

Example sentences for: eluted

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Following incubation at room temperature for 10 min, the bound proteins were eluted with buffer A plus 1 M imidazole, and then analyzed by SDS-PAGE and Western blot, as and where mentioned.
The eluted fractions were spun through 0.22 μM Millex filters (Millipore), dialyzed for 6 hours at 4°C in dialysis buffer (20 mM Tris (pH 7.9), 100 mM KCl, 20% (v/v) glycerol, and 1 mM DTT), frozen in liquid nitrogen, and stored at -80°C.
The PCR products were purified with Qiagen PCR Purification Kit (Qiagen, Valencia, CA) and eluted into 42 μl of ddH 2 O, which was used for indirect labeling with PCR.
The 120 kDa and 140 kDa bands likely represent the β1 and α5 subunits (as above), while the 150 kDa band may represent either the α2 or α3 integrin subunits, which are known to be expressed by VSMCs [ 26 ] . Affinity chromatography on the control III 11-C column yielded no radioiodinated proteins that were eluted from the column (Fig.
Remaining proteins were then re-precipitated from the eluate using mAb directly conjugated to sepharose 4B beads for 30 min at 4°C, washed three times with 1% Triton X100 in PBS, eluted with Laemmli sample buffer, and resolved on SDS-PAGE.