Words similar to eluted

Example sentences for: eluted

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The eluted fractions were spun through 0.22 μM Millex filters (Millipore), dialyzed for 6 hours at 4°C in dialysis buffer (20 mM Tris (pH 7.9), 100 mM KCl, 20% (v/v) glycerol, and 1 mM DTT), frozen in liquid nitrogen, and stored at -80°C.
The insect-selective toxin was loaded onto the C18 column and eluted in a linear gradient of 10-60 % Solvent B (95% acetonitrile; 0.1% TFA) over 60 min at a flow rate of 100 μl/min.
The 120 kDa and 140 kDa bands likely represent the β1 and α5 subunits (as above), while the 150 kDa band may represent either the α2 or α3 integrin subunits, which are known to be expressed by VSMCs [ 26 ] . Affinity chromatography on the control III 11-C column yielded no radioiodinated proteins that were eluted from the column (Fig.
The purified proteins were eluted with reduced glutathione at 1 mg/ml in elution buffer (100 mM Tris (pH 7.9), 120 mM NaCl).
A later study by these authors identified 120-kDa and 115-kDa APNs coeluting from an anion-exchange column that together yielded a Cry1Aa affinity of 53 nM [ 10 ] . These APNs eluted just prior to a 120 kDa APN with 7.6 nM affinity.