Words similar to eluted

Example sentences for: eluted

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Resin was washed with a low concentration of competing FLAG peptide (Sigma; 10 μg/ml in TBSE), then the column was eluted with 2 ml of competing FLAG peptide at 120 μg/ml in TBSE.
First-strand cDNA was purified from unincorporated amino allyl-dUTPs on QIAquick PCR purification columns (Qiagen, Valencia, CA) according to manufacturer's instructions, except that QIAquick wash buffer was replaced with 5 mM K +phosphate buffer (pH 8.5) containing 80% ethanol, and cDNA was eluted with 4 mM K +phosphate buffer (pH 8.5).
It was then loaded on a 10 cm × 1 cm hydroxylapatite (BioGel HTP, BioRad) column and eluted under pressure with a 0-600 mM phosphate gradient (200 mL total volume, flow rate 0.5 ml/min).
1C, Pop2p, together with SCF core subunits, eluted in fractions corresponding to 400 - 600 kDa, irrespective of whether Pop1p was present or not (Fig.
Highly purified GFP-BLM eluted in Buffer B + 200 mM imidazole and was stored at -70°C.