Example sentences for: elution

How can you use “elution” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To further fractionate the proteins present in the partially purified GSTCdc6 fraction the glutathione column was washed with 200 mM and 300 mM sodium chloride prior to GSTCdc6 elution with reduced glutathione.

  • Sera from rabbits immunized with a killed preparation of M. paratuberculosis (1-2 ml) were passed over the column followed by three washes and elution according to the instructions of the manufacturer.

  • Protein in positive elution fractions was refolded to native structure by serial dialysis against buffer D (20 mm HEPES, 0.1 M KCl, 0.2 mm EDTA, 0.5 mm dithiothreitol, 0.5 mm phenylmethylsulfonyl fluoride, 20% glycerol) containing stepwise decreasing concentrations of urea (6 M for 2 hours, 4 M for 2 hours, 2 M for 2 hours, no urea for 12 hours).

  • The mRNA was then suspended in 20 μl of elution buffer and the concentration was determined by measuring the attenuance at a wavelength of 260 nm.

  • Proteins were eluted using reduced glutathione elution buffer (50 mM Tris.Cl, pH 8.0, 20 mM reduced glutathione, 0.01% Nonidet P-40, 100 mM NaCl).


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