Words similar to elution
Example sentences for: elution
How can you use “elution” in a sentence? Here are some example sentences to help you improve your vocabulary:
The reaction was halted by the addition of 2 μL of 0.5 M EDTA pH 8.0, and product isolated with the MinElute Reaction Cleanup Kit (Qiagen), increasing the elution volume to 20 μL.
PcrG was present in the elution fraction (Fig.
Reactions were neutralized with 10 μl of 1 M HCl, and cDNA was purified on QIAquick columns (Qiagen) according to the manufacturer's directions but substituting phosphate wash buffer (5 mM potassium phosphate pH 8.0, 80% ethanol) for buffer PE, and phosphate elution buffer (4 mM potassium phosphate pH 8.5) for buffer EB.
Protein in positive elution fractions was refolded to native structure by serial dialysis against buffer D (20 mm HEPES, 0.1 M KCl, 0.2 mm EDTA, 0.5 mm dithiothreitol, 0.5 mm phenylmethylsulfonyl fluoride, 20% glycerol) containing stepwise decreasing concentrations of urea (6 M for 2 hours, 4 M for 2 hours, 2 M for 2 hours, no urea for 12 hours).
HA-NFATp was purified from baculovirus-infected Hi-five cell extracts using anti-HA affinity chromatography followed by elution with an HA-epitope peptide (Figure 1B).