Example sentences for: elution

How can you use “elution” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The reaction was halted by the addition of 2 μL of 0.5 M EDTA pH 8.0, and product isolated with the MinElute Reaction Cleanup Kit (Qiagen), increasing the elution volume to 20 μL.

  • PcrG was present in the elution fraction (Fig.

  • Reactions were neutralized with 10 μl of 1 M HCl, and cDNA was purified on QIAquick columns (Qiagen) according to the manufacturer's directions but substituting phosphate wash buffer (5 mM potassium phosphate pH 8.0, 80% ethanol) for buffer PE, and phosphate elution buffer (4 mM potassium phosphate pH 8.5) for buffer EB.

  • Protein in positive elution fractions was refolded to native structure by serial dialysis against buffer D (20 mm HEPES, 0.1 M KCl, 0.2 mm EDTA, 0.5 mm dithiothreitol, 0.5 mm phenylmethylsulfonyl fluoride, 20% glycerol) containing stepwise decreasing concentrations of urea (6 M for 2 hours, 4 M for 2 hours, 2 M for 2 hours, no urea for 12 hours).

  • HA-NFATp was purified from baculovirus-infected Hi-five cell extracts using anti-HA affinity chromatography followed by elution with an HA-epitope peptide (Figure 1B).


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