Words similar to ecac
Example sentences for: ecac
How can you use “ecac” in a sentence? Here are some example sentences to help you improve your vocabulary:
It needs to be pointed out, however, that since Caco-2 cells are not derived from normal human intestinal tissue, it is possible that ECaC1 could be expressed in the human intestine.
Based on new sequence information (GenBank accession number AF304464 (ECaC1) and AF365927 (CaT1), [ 25 ] ) the nucleotides determined following the sequencing of our PCR product from Caco-2 cells was an identical match for CaT1 rather than ECaC1 (data not shown).
As described in the Method section, a 259 bp PCR product was generated with a second set of PCR primers that would specifically encompass a differential Bgl1 restriction enzyme cut site present in CaT1 but not ECaC1.
However, our observation in Caco-2 cells is consistent with the recent report by Barley and colleagues [ 16 ] indicating a high expression level of CaT1/ECaC2 in human intestine based on Northern blotting, and similar findings by Peng et al.
Second, the sequences of the coding region of the CaT1 and ECaC1 genes (GenBank AF365927 and AF304464) indicate that an additional identification step based on restriction fragment length polymorphism (RFLP) analysis could also be used to confirm the identity of the CaT1 mRNA in Caco-2 cells.