Example sentences for: ecac

How can you use “ecac” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These primers yield a 259 bp PCR product with a specific restriction site that makes it possible to clearly distinguish between CaT1 and ECaC1 using the restriction enzymes Bgl1 (New England Biolabs).

  • The DNA sequencing facility at Tufts University sequenced the PCR product to identify whether the amplified PCR product from Caco-2 cells was either CaT1 or the closely related ECaC1 sequence.

  • In this PCR amplified product, there is no predicted Bgl1 restriction enzyme site in the corresponding ECaC1 sequence.

  • However, our observation in Caco-2 cells is consistent with the recent report by Barley and colleagues [ 16 ] indicating a high expression level of CaT1/ECaC2 in human intestine based on Northern blotting, and similar findings by Peng et al.

  • Second, the sequences of the coding region of the CaT1 and ECaC1 genes (GenBank AF365927 and AF304464) indicate that an additional identification step based on restriction fragment length polymorphism (RFLP) analysis could also be used to confirm the identity of the CaT1 mRNA in Caco-2 cells.


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