Words similar to ecac
Example sentences for: ecac
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Intestinal calcium absorption is increased after feeding a low calcium diet or under conditions of increased calcium needs, such as during growth, via a parathyroid hormone (PTH)-vitamin D mediated process [ 3 ] . A lowering of serum calcium levels stimulates the secretion of PTH that then activates the renal conversion of inactive 25-hydroxyvitamin D to the active hormonal metabolite 1,25-dihydroxyvitamin D. At the intestine, 1,25-dihydroxyvitamin D, acting through a nuclear receptor, stimulates transcription of vitamin D-mediated genes, including calbindin D, involved in calcium transport thereby acting as the primary hormonal regulator of active intestinal calcium transport [ 3 ] . It is thus likely that any putative key molecular player in calcium absorption, such as CaT1 or ECaC1, should be a prime target for hormonal regulation by 1,25-dihydroxyvitamin D. However, to date, neither CaT1 nor ECaC1 expression has been shown to be related to serum 1,25-dihydroxyvitamin D or vitamin D status [ 10 16 ] .
The cDNA solution containing 0.1 μg equivalent RNA was analyzed by PCR for GAPDH, human calbindin D 9K , and human CaT/ECaC.
This is important because it has been suggested that because of the high degree of sequence similarity (97%) between the CaT1 and ECaC1 genes care should be taken in interpreting data obtained by hybridization, PCR or other methodologies based on nucleotide sequences [ 24 ] . First, the PCR product derived from the putative ECaC1 or CaT1 gene was purified and sequenced.
As described in the Method section, a 259 bp PCR product was generated with a second set of PCR primers that would specifically encompass a differential Bgl1 restriction enzyme cut site present in CaT1 but not ECaC1.
Thus, based on this collective information, we conclude that CaT1, but not ECaC1, is expressed in Caco-2 cells.
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