Words similar to ecac
Example sentences for: ecac
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Intestinal calcium absorption is increased after feeding a low calcium diet or under conditions of increased calcium needs, such as during growth, via a parathyroid hormone (PTH)-vitamin D mediated process [ 3 ] . A lowering of serum calcium levels stimulates the secretion of PTH that then activates the renal conversion of inactive 25-hydroxyvitamin D to the active hormonal metabolite 1,25-dihydroxyvitamin D. At the intestine, 1,25-dihydroxyvitamin D, acting through a nuclear receptor, stimulates transcription of vitamin D-mediated genes, including calbindin D, involved in calcium transport thereby acting as the primary hormonal regulator of active intestinal calcium transport [ 3 ] . It is thus likely that any putative key molecular player in calcium absorption, such as CaT1 or ECaC1, should be a prime target for hormonal regulation by 1,25-dihydroxyvitamin D. However, to date, neither CaT1 nor ECaC1 expression has been shown to be related to serum 1,25-dihydroxyvitamin D or vitamin D status [ 10 16 ] .
CaT1 and ECaC1 are found in several tissues, including 1,25-dihydroxyvitamin D-responsive epithelia in the intestine and kidney.
However, our observation in Caco-2 cells is consistent with the recent report by Barley and colleagues [ 16 ] indicating a high expression level of CaT1/ECaC2 in human intestine based on Northern blotting, and similar findings by Peng et al.
These primers yield a 259 bp PCR product with a specific restriction site that makes it possible to clearly distinguish between CaT1 and ECaC1 using the restriction enzymes Bgl1 (New England Biolabs).
In addition, further attempts at PCR amplification of Caco-2 cell RNA with other PCR primers designed to specifically amplify ECaC1 did not result in an amplified product under our conditions (data not shown).
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