Words similar to ecac
Example sentences for: ecac
How can you use “ecac” in a sentence? Here are some example sentences to help you improve your vocabulary:
In the current paper, we have used the Caco-2 cell culture model to demonstrate that CaT1 mRNA is preferentially expressed in the human enterocyte compared to ECaC1, and for the first time demonstrate that 1,25-dihydroxyvitamin D treatment can cause a rapid and marked increase in CaT1 mRNA expression in the enterocyte.
These primers yield a 259 bp PCR product with a specific restriction site that makes it possible to clearly distinguish between CaT1 and ECaC1 using the restriction enzymes Bgl1 (New England Biolabs).
Based on new sequence information (GenBank accession number AF304464 (ECaC1) and AF365927 (CaT1), [ 25 ] ) the nucleotides determined following the sequencing of our PCR product from Caco-2 cells was an identical match for CaT1 rather than ECaC1 (data not shown).
This is important because it has been suggested that because of the high degree of sequence similarity (97%) between the CaT1 and ECaC1 genes care should be taken in interpreting data obtained by hybridization, PCR or other methodologies based on nucleotide sequences [ 24 ] . First, the PCR product derived from the putative ECaC1 or CaT1 gene was purified and sequenced.
CaT1 and ECaC1 are found in several tissues, including 1,25-dihydroxyvitamin D-responsive epithelia in the intestine and kidney.
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