Words similar to ec-
Example sentences for: ec-
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All 12 sequences in this region have high similarity to the derived consensus sequence GXGXXG(7X)A(SX)GXGXXG(4X)D(9X)R, which defines a nucleotide binding motif [ 16 25 ] . In addition, all sequences have five residues, or conservative substitutions in the case of Pa-MoeB, shown to participate in the adenylation reaction catalyzed by Ec-MoeB (Figure 3) [ 7 ] . The fact that such high similarities are retained in the ThiF coding region across three bacterial divisions suggests that this domain functions more or less autonomously.
Ec-MoeB, Mt-MoeBR, Pa-MoeB, Pa-MoeBR, and Sc-MoeBR all contain two pairs of cysteine residues, each arranged in a CXXC motif and spaced 68-70 residues apart, like those in ThiF proteins (arrows in Figures 2cand 3).
MoeB, as represented by the functionally characterized Ec-MoeB, consists of an N-terminal ThiF domain followed by a 2X CXXC domain with both CXXC pairs intact and no rhodanese-like domain.
MoeBR has the ThiF and 2X CXXC domains configured like MoeB but includes an N-terminal rhodanese-like domain, the polyproline motif, and has a Y substitution at position 155 (Ec-MoeB numbering).
In Ec-MoeB, β sheet 5 (residues 149-155 of Ec-MoeB, Figure 3) forms part of the pocket which accepts the C-terminal portion of MoaD, of which the terminal glycine is subsequently adenylated [ 7 ] . Ec-MoeB I155, located at the C-terminal end of this beta loop, is substituted in MoeBR proteins by Y and in MoeZ proteins by F or Y. These bulky substitutions may restrict access of the C-terminus of MoeD-like proteins and may indicate that a different type of substrate is adenylated by MoeBR and MoeZ.
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