Words similar to ecr

Example sentences for: ecr

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Cells were simultaneously co-transfected by electroporation (Gene Pulser, Bio-Rad Inc.) with pGAL4-EcR, pVP16-mRXR, pRL-TK (Renilla luciferase) and pGAL4-EGFP.
A description of the plasmids encoding the two components of an ecdysone inducible transcription activation system was reported previously [ 14 ] . The plasmids pGAL4-EcR and pVP16-mRXR encode proteins which heterodimerize and regulate transcription upon the addition of ecdysone type ligands (Figure 1A).
[ 7 ] This system is comprised of a chimeric protein composed of the VP16 activation domain (Vg) fused to an ecdysone receptor (EcR) with altered DNA-binding specificity heterodimerizes with the retinoid X receptor (RXR) and binds a unique synthetic response element not recognized by natural nuclear hormone receptors.
MBT-2 cells, a mouse urothelial cancer cell line, or Panc02, a mouse ductal adenocarcinoma, were transfected by electroporation with a mixture of the four plasmids; the two components of the gene expression system, pVP16-mRXR and pGAL4-EcR; the indicator and selection plasmid, pGAL4-EGFP-SV40-neo; and the internal control plasmid, pRL-TK.
The plasmids pGAL4-EcR encodes a fusion protein between the yeast GAL4 DNA binding domain and the ligand binding domain of the ecdysone receptor from the spruce budworm, Choristoneura occidentalis.