Words similar to dutp
Example sentences for: dutp
How can you use “dutp” in a sentence? Here are some example sentences to help you improve your vocabulary:
CMV = cytomegalovirus, MCMV = murine cytomegalovirus, MCP-1 = monocyte chemoattractant protein-1, PCNA = proliferating cell nuclear antigen, PDGF = platelet derived growth factor, SSc = systemic sclerosis, TGF-β 1 = transforming growth factor-β 1 , TUNEL = terminal TdT-mediated dUTP nick end labeling, α-SMA = α-smooth muscle actin
The following reagents were added: 6 μl of 5X first strand buffer, 3 μl 0.1 M DTT, 0.7 μl 50X dNTP (25 mM dATP, dCTP, dGTP and 10 mM dTTP), 3 μl 1 mM Cy3-dUTP or Cy5-dUTP and 1.7 μl Superscript™ II (200 U/μl).
For each hybridization, 2 μg of each purified sample of polyadenylated RNA was reverse-transcribed in the presence of fluorescently labeled nucleoside triphosphates (Cy3-dUTP for reference sample, Cy5-dUTP for experimental sample).
The induction of apoptosis was determined by means of the TUNEL (deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay using a kit supplied by Promega (Madison, WI, USA).
BSA = Bovine serum albumin; CSC = cigarette smoke condensate; ERK = extracellular signal-regulated kinase; GM-CSF = granulocyte-macrophage colony-stimulating factor; HBE = human bronchial epithelial cell; IL = interleukin; IκB = inhibitor of NFκB; MAPK = mitogen-activated protein kinase; MEK-1 = MAPK kinase; NFκB = nuclear factor-kappa B; NHBE = normal human bronchial epithelial cell; PBS = phosphate-buffered saline; PDTC = pyrrolidine dithiocarbamate; RT-PCR = reverse transcriptase-polymerase chain reaction; sICAM = soluble intercellular adhesion molecule; TNF = tumour necrosis factor; TUNEL = deoxynucleotidyl transferase-mediated dUTP nick end labeling
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