Words similar to dutp
Example sentences for: dutp
How can you use “dutp” in a sentence? Here are some example sentences to help you improve your vocabulary:
To provide an internal standard for each measurement, a pool of reference mRNA (labeled with Cy-3 dUTP, 'green') derived from combining mRNA from 10 different human cell lines was mixed with the Cy-5 labeled experimental samples before hybridization with the cDNA microarray.
To investigate hybridization consistency further, aliquots of mRNAs from immature ears (IME) and 4DR were each labeled separately with Cy3-dUTP or Cy5-dUTP.
Total RNA (100 μg) was fluorescence-labeled by oligo-dT-primed reverse transcription to cDNA in the presence of Cy3-dUTP as described by Eisen and Brown [ 31].
The following reagents were added: 6 μl of 5X first strand buffer, 3 μl 0.1 M DTT, 0.7 μl 50X dNTP (25 mM dATP, dCTP, dGTP and 10 mM dTTP), 3 μl 1 mM Cy3-dUTP or Cy5-dUTP and 1.7 μl Superscript™ II (200 U/μl).
The two gene fragments were labelled separately with digoxigenin-11-dUTP (Boehringer Mannheim, Germany) by nick-translation (Life Technologies Inc., Gaithersburg, MD).
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