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Example sentences for: dutp
How can you use “dutp” in a sentence? Here are some example sentences to help you improve your vocabulary:
In situ detection of DNA fragmentation was performed by incorporation of fluorescein-12-dUTP at the 3'-OH ends of DNA using Terminal deoxynucleotidyl Transferase (TUNEL assay) (Apoptosis Detection System, Fluorescein, Promega, Madison, WI), as performed previously by this laboratory [ 7 26 ] . Nuclei were counterstained using propidium iodide (Sigma Chemical Co., St. Louis, MO).
To further investigate if cell death occurred in the developing iridocorneal angle, we used a fluorescent double labeling assay that identifies fragmented DNA using fluorescently labeled dUTP and detects chromatin condensation by binding of the dye YOYO-1.
AGTII, angiotensin II; BSA, bovine serum albumin; CHX, cycloheximide; CPT, camptothecin; DMSO, dimethyl sulfoxide; FITC, fluorescein isothiocyanate; HBSS, Hanks' balanced salt solution; HUVEC, human umbilical vein endothelial cells; IC-IgG, IgG isotype control; Mab, monoclonal antibody; MP, microparticles; PE, phycoerythrin; PI, propidium iodide; PMA, phorbol-myristate-acetate; PS, phosphatidylserine; TRAP, thrombin receptor activating peptide (SFLLRNP); TNF, tumor necrosis factor; TPA, tissue plasminogen activator; TUNEL, terminal deoxynucleotidyltransferase dUTP nick end labeling; ZVAD, Z-Val-Ala-Asp-fluoromethyl ketone.
Amino-allyl dUTP was incorporated during the PCR step as described in [ 22 ] , and at http://www.microarrays.org.
cDNA from experimental samples was generated by reverse transcription in the presence of Cy-5 dUTP, and was mixed with a reference pool of cDNA derived from 10 different cell lines (Brown laboratory control reference 'E'), labeled with Cy-3 dUTP.
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