Words similar to dutp
Example sentences for: dutp
How can you use “dutp” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RT mix included 5.6 μl of 5X RT buffer, 0.02 mM of dNTPs (with dTTP, not dUTP, Perkin Elmer, Foster City, CA), 14.
Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) (In situ Cell Death Detection Kit, POD, Boehringer-Manheim).
The PCR mix per reaction included 10 μl SYBR Green buffer, 1.5 mM MgCl 2 , 1 μM dNTP mix (with dUTP), 0.5 μl CMV primer mix, 2.5 units Ampli Taq gold, 0.05 units of uracil-N-glycosylase (UNG, Epicentre, Madison, WI) that had been diluted 1:10 in UNG buffer, and molecular grade water to bring the volume up to 45 μl with the addition of 5 μl of cDNA or standard.
BAC DNA (1 μg) was labeled with biotin-16-dUTP (Roche) or digoxigenin-11-dUTP (Roche) by nick translation.
DNA fragmentation was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, using fluorescein-12-dUTP, according to the instructions supplied by the manufacturer (Promega Corp., Madison, WI).
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