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Example sentences for: dutp

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In situ detection of DNA fragmentation was performed by incorporation of fluorescein-12-dUTP at the 3'-OH ends of DNA using Terminal deoxynucleotidyl Transferase (TUNEL assay) (Apoptosis Detection System, Fluorescein, Promega, Madison, WI), as performed previously by this laboratory [ 7 26 ] . Nuclei were counterstained using propidium iodide (Sigma Chemical Co., St. Louis, MO).
cDNA from experimental samples was generated by reverse transcription in the presence of Cy-5 dUTP, and was mixed with a reference pool of cDNA derived from 10 different cell lines (Brown laboratory control reference 'E'), labeled with Cy-3 dUTP.
5-5 μg) obtained after two rounds of amplification was labeled in a reverse-transcription reaction in the presence of Cy3-labeled (reference sample) or Cy5 (test sample)-labeled dUTP (Amersham, Piscataway, NJ).
The following reagents were added: 6 μl of 5X first strand buffer, 3 μl 0.1 M DTT, 0.7 μl 50X dNTP (25 mM dATP, dCTP, dGTP and 10 mM dTTP), 3 μl 1 mM Cy3-dUTP or Cy5-dUTP and 1.7 μl Superscript™ II (200 U/μl).
To provide an internal standard for each measurement, a pool of reference mRNA (labeled with Cy-3 dUTP, 'green') derived from combining mRNA from 10 different human cell lines was mixed with the Cy-5 labeled experimental samples before hybridization with the cDNA microarray.