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Example sentences for: dutp

How can you use “dutp” in a sentence? Here are some example sentences to help you improve your vocabulary:

BSA = Bovine serum albumin; CSC = cigarette smoke condensate; ERK = extracellular signal-regulated kinase; GM-CSF = granulocyte-macrophage colony-stimulating factor; HBE = human bronchial epithelial cell; IL = interleukin; IκB = inhibitor of NFκB; MAPK = mitogen-activated protein kinase; MEK-1 = MAPK kinase; NFκB = nuclear factor-kappa B; NHBE = normal human bronchial epithelial cell; PBS = phosphate-buffered saline; PDTC = pyrrolidine dithiocarbamate; RT-PCR = reverse transcriptase-polymerase chain reaction; sICAM = soluble intercellular adhesion molecule; TNF = tumour necrosis factor; TUNEL = deoxynucleotidyl transferase-mediated dUTP nick end labeling
Amino-allyl dUTP was incorporated during the PCR step as described in [ 22 ] , and at http://www.microarrays.org.
For each sample, 50 μL solution containing approximately 5 units of Klenow large fragment (New England Biolabs, Beverly, MA), 3 μg of single stranded binding protein (US Biochemicals, Cleveland, OH), and 0.5 μM Cy3- or Cy5-labeled dATP, dCTP, dGTP or dUTP (Perkin Elmer) was pipeted onto the gel.
The RT mix included 5.6 μl of 5X RT buffer, 0.02 mM of dNTPs (with dTTP, not dUTP, Perkin Elmer, Foster City, CA), 14.
To examine the consistency of experiments from the labeling reactions through to the scanning process, a pool of mRNA from 4-day-old roots (4DR) was used to synthesize two fluorescent cDNA targets using Cy3-dUTP or Cy5-dUTP.