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Example sentences for: dutp

How can you use “dutp” in a sentence? Here are some example sentences to help you improve your vocabulary:

For each hybridization, 2 μg of each purified sample of polyadenylated RNA was reverse-transcribed in the presence of fluorescently labeled nucleoside triphosphates (Cy3-dUTP for reference sample, Cy5-dUTP for experimental sample).
In situ detection of DNA fragmentation was performed by incorporation of fluorescein-12-dUTP at the 3'-OH ends of DNA using Terminal deoxynucleotidyl Transferase (TUNEL assay) (Apoptosis Detection System, Fluorescein, Promega, Madison, WI), as performed previously by this laboratory [ 7 26 ] . Nuclei were counterstained using propidium iodide (Sigma Chemical Co., St. Louis, MO).
cDNA from experimental samples was generated by reverse transcription in the presence of Cy-5 dUTP, and was mixed with a reference pool of cDNA derived from 10 different cell lines (Brown laboratory control reference 'E'), labeled with Cy-3 dUTP.
To investigate hybridization consistency further, aliquots of mRNAs from immature ears (IME) and 4DR were each labeled separately with Cy3-dUTP or Cy5-dUTP.
5-5 μg) obtained after two rounds of amplification was labeled in a reverse-transcription reaction in the presence of Cy3-labeled (reference sample) or Cy5 (test sample)-labeled dUTP (Amersham, Piscataway, NJ).