Words similar to dttp

Example sentences for: dttp

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Reactions were carried out at 37°C for 2 min at 20 μM dTTP concentration and quenched with Sanger's gel loading dye [ 35 ] . The reaction products were resolved by denaturing polyacrylamide-urea gel electrophoresis and analyzed on a PhosphorImager (Molecular Dynamics, Inc.).
The following reagents were added: 6 μl of 5X first strand buffer, 3 μl 0.1 M DTT, 0.7 μl 50X dNTP (25 mM dATP, dCTP, dGTP and 10 mM dTTP), 3 μl 1 mM Cy3-dUTP or Cy5-dUTP and 1.7 μl Superscript™ II (200 U/μl).
The RT mix included 5.6 μl of 5X RT buffer, 0.02 mM of dNTPs (with dTTP, not dUTP, Perkin Elmer, Foster City, CA), 14.
Up to 50 ng of CIP-treated template DNA were incubated for 20 minutes at 37°C in a 10 μL solution containing 20 U TdT (NEB), 0.2 M potassium cacodylate, 25 mM Tris-HCl pH 6.6, 0.25 mg/ml BSA, 0.75 mM CoCl 2 , 4.6 μM dTTP and 0.4 μM ddCTP.
Subsequently, 25 μl of a premix containing 2 μl of 100 mM DTT (dithiothreitol), 10 μl 5X Superscript II buffer, 1 μl 50X aadNTP mix (25 mM dA, C, and GTP, 8.4 mM dTTP, and 16.